I= PROTEIN CHANGES IN SENILE CATARACT FORMATION
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Title of Thesis
PROTEIN CHANGES IN SENILE CATARACT FORMATION

Author(s)
Shamshad Zarina
Institute/University/Department Details
H.E.J. Research institute of Chemistry/ University of Karachi
Session
1991
Subject
Chemistry
Number of Pages
87
Keywords (Extracted from title, table of contents and abstract of thesis)
senile, cataract, visual disability, lens opacity, lens proteins, aqueous humor, plasma, immature, cataract nigra, lens, chromatography, enzymatic cleavage, chemical cleavage

Abstract
Lens opacity i.e. cataract, is one of the major causes of visual disability and blindness. The distribution of cataract varies widely and the age related or senile cataract is common all over the world. Over seventeen million people in the whole world are effected by the loss of lens transparency, of which two million persons belong to Pakistan. Thus the prevention of cataract requires a complete understanding of the lens and its structure so that possible preventive measures could be development. Present study was directed towards exploring biochemical causes of lens opacity by studying lens proteins, aqueous humor and plasma.

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623.75 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
82.73 KB
2 1 Introduction 1
142.77 KB
  1.1 General Introduction 1
  1.2 Lens 1
  1.3 Cataract 8
  1.4 Aqueous Humor 15
  1.5 Plasma 16
  1.6 Objectives Of Present Study 18
3 2 Experimental 19
69.28 KB
  2.1 Sample Collection 19
  2.2 Classification Lenses 19
  2.3 Isolation Of Proteins 19
4 3 Results 27
137.08 KB
  3.1 Solubility Of Lens Proteins 27
  3.2 Gel Chromatography 30
  3.3 High Performance Liquid Chromatography 30
  3.4 Electrophoresis 30
  3.5 Chemical Cleavage 30
  3.6 Enzymatic Cleavage 45
  3.7 Amino Acid Analysis 45
  3.8 Sequencing 45
5 4 Discussion 51
142.86 KB
  4.1 Low Molecular Mass Lens Proteins 51
  4.2 Comparison Of Lens Protein In Different Stages Of Cataract 63
  4.3 Aqueous Humor And Plasma 65
  4.4 Conclusion 68
6 5 References
177.06 KB