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AMINO ACID SEQUENCE OF THE HEMOGLOBIN FROM THE REPTILE UROMASTIX HARDWICK II

Naqvi, Sabira (1984) AMINO ACID SEQUENCE OF THE HEMOGLOBIN FROM THE REPTILE UROMASTIX HARDWICK II. PhD thesis, University of Karachi, Karachi.

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Abstract

This is the first study on the hemoglobin of Uromastix Hardwick. We are reporting the amino acid sequence of its hemoglobin. The separation of hemoglobin components and polypeptide chains were carried out on various columns and purity could not be achieved. The globin was, therefore carboxmethylated and cleaved with trypsin. The tryptic peptides were separated by gel filtration and further purified by reverse phase-high performance liquid chromatography. The amino acid composition of the peptides were determined after hydrolysis. The amino acid sequence of purified peptides was determined manually by the Adman degradation and DABITC method. The sequence of the peptides in the α and β chain was deduced from their homology with known sequence of ht polypeptides chains of other species. The sequence of Uromastix α- chain is compared with the α- chain of crocodile, viper, human and chicken, while the β-chain is compared with crocodile , human and chicken β-chain. The results show that hemoglobin chains are homologous with those of birds and mammals and the relationship hardly appear closer to known sequences of reptiles. The results also show chain heterogeneity because at least two types of both α and β chain are present in the hemoglobin studies

Item Type:Thesis (PhD)
Uncontrolled Keywords:amino acid, hemoglobin, uromastix hardwick ii, peptides, respiratory function, reptilian hemoglobin, cm-cellulose
Subjects:Physical Sciences (f) > Chemistry(f2)
ID Code:930
Deposited By:Mr. Muhammad Asif
Deposited On:24 Nov 2006
Last Modified:04 Oct 2007 21:03

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