I= STUDIES OF THE CHEMICAL CONSTITUENTS OF GUALACUM OFFICINALEL
Pakistan Research Repository Home
 

Title of Thesis
STUDIES OF THE CHEMICAL CONSTITUENTS OF GUALACUM OFFICINALEL

Author(s)
Nikhat Saba
Institute/University/Department Details
H.E.J Research Institute of Chemistry/ University of Karachi
Session
1996
Subject
Chemistry
Number of Pages
232
Keywords (Extracted from title, table of contents and abstract of thesis)
gualacum officinalel, zygophyllaceae, tritrpenoid saponins, guaianin m, guaianin n, guaianin o, guaianin p, guaianin q, guaianin r

Abstract
Guaiacum officinale L. (Zygophyllaceae) is a medicinal and ornamental plant. It is commonly cultivated in the gardens in Pakistan.

The present thesis describes the isolation and structure elucidation of six new tritrpenoid saponins namely guaianin M, guaianin N, guaianin O, guaianin P, guaianin Q, guaianin R as well as known saponin guaianin S (akebia saponin PE) form Guaiacum officinale L. Guaianins M, N, O and S were isolated fro the flowers while guaianin P, Q, and R were isolation for the bark of the plant.

Both the flowers and bark were extracted in methanol and evaporated to gum separately. The gummy residues were partitioned between water and ethyl acetate and then water and butanol butanoilc extract contained saponins. Sapoinns were purified by VLC column chromatography, flash chromatography, and finally by HPLC through semi preparative RP-18 column.

The structures of the saponins were elucidated with the help of chemical as well as spectroscopical analysis of the compound. The later included extensive studies of various techniques of both 1-D and 2-D NMR spectroscopes.

From flowers we isolated a number of reported compounds. Besides them only three were new and one was reported for the first time form this plant. From the bark we isolated a number of reported compounds besides three new compounds guaianin P, Q and R. The chemical names and structures of guaianin M, N, O, P, Q,R and S are given below.

Download Full Thesis
1490.95 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents/ Summary
90.39 KB
2 1 Introduction 10
131.28 KB
  1.1 Introduction Of Saponin 14
  1.2 Introduction Of Plant 17
  1.3 Pharmacological, Biological And Medicinal Uses Of Saponins 18
3 2 Biosynthesis 27
137.06 KB
  2.1 Primary And Secondary Metabolites 28
  2.2 Classification Of Terpenes 29
  2.3 General Biosynthesis Of Terpenes 30
  2.4 Biosynthesis Of Teriterpenoids 35
  2.5 Biosynthesis Of Saponins 46
4 3 Literature Survey Of Guaiacum Officinalel 48
211.11 KB
  3.1 Introduction Of The Plant Of G. Officinale 49
  3.2 Pharmacological Screening Of G. Officinale L 50
  3.3 Phytochemsitry Of G. Officinale 51
5 4 Present Work 86
904.97 KB
  4.1 Extraction 87
  4.2 Isolation 89
  4.3 Elucidation Of Structure Of Saponins 93
  4.4 Isolation And Structure Elucidation Of Guaianin M 105
  4.5 Isolation And Structure Elucidation Of Guaianin N 114
  4.6 Isolation And Structure Elucidation Of Guaianin O 126
  4.7 Isolation And Structure Elucidation Of Guaianin P 141
  4.8 Isolation And Structure Elucidation Of Guaianin Q 158
  4.9 Isolation And Structure Elucidation Of Guaianin R 170
  4.10 Isolation And Structure Elucidation Of Guaianin S 187
6 5 Experimental 195
164.72 KB
  5.1 Characterization Of Guaianin M 199
  5.2 Characterization Of Guaianin N 201
  5.3 Characterization Of Guaianin O 203
  5.4 Characterization Of Guaianin P 206
  5.5 Characterization Of Guaianin Q 209
  5.6 Characterization Of Guaianin R 211
  5.7 Characterization Of Guaianin S 214
7 6 References 216
112.98 KB
8 7 List Of Publications 231
13.38 KB