I= GENETIC STUDIES ON ISOZYME PATTERNS IN TRITICUM AESTIVUM L
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Title of Thesis
GENETIC STUDIES ON ISOZYME PATTERNS IN TRITICUM AESTIVUM L

Author(s)
Farzana Nasir Naqvi
Institute/University/Department Details
University of Karachi/ Department of Genetics
Session
1997
Subject
Genetics
Number of Pages
290
Keywords (Extracted from title, table of contents and abstract of thesis)
triticum aestivum l, wheat, rye, barley, isoperoxidases, isoamylases, isozymes, hexaploid, teraploid, diploid, aegllops, . cylinrica, ae. ovata, e. biuncialls, ae. crassa, e. juvenalls, ae. vavilvi, e. triaristata

Abstract
The tribe Triticeae includes three important cereals namely wheat, rye and barley and their wild relatives. Available wheat cultivars have limited genetic variability for resistance to insects, pests and diseases. They also lack tolerance to salinity , drought and other abiotic stresses. In the light of growing evidence of the limited and narrowing genetic pool of advanced wheat cultivars, research on the genetic resources of wild relatives of cultivated wheat becomes increasingly important.

Present studies were undertaken with aim to evaluate variability of isoperoxidases and isoamylases in different tissues of cultivated wheat and its related species. Determination of the chromosomal locations of genes controlling these isozymes was also carried out. The ultimate aim of the studies was to establish biochemical marker(s) characterizing genetic resistance.

Current work is an indicative of homology of isoperoxidases and isoamylases at various polity levels of wild and cultivated wheats and their relatives. However, differences in relative mobility and absence or presence of certain bands had been noticed among hexaploid, teraploid and diploid wheats and Aegllops. Results revealed the presence of certain isoperoxidases and isoamylases in Aegilops species which were absent in most of hexaploid wheat accessions. These isozymes could be used to differentiate species of Triticum.

Comparison of hexaploid wheat isozymes with progenitors exhibited the presence of certain common bands between diploid, tetraploid and hexaploid species which is an indicative of the contribution of the respective genomes to hexaploid wheat.

Appearance of bands at similar sites in synthetic hexaploid wheat of ‘CS’ strengthen the evidence about the origin of their genome. Variation observed for few bands between natural wheats and synthetic wheat exploited for the improvement of bread wheat.

Amphiploid T aestivum – Ae. Ovata exhibited the qualitative pattern of both parents, however, specific band of Ae, ovata had also been noticed.

Aneuploid lines of T, aestivum L. cv. ‘Chinese Spring’ alongwith its disomic control (2n=6x=42: AABBDD) were utilized to locate the genes for leaf and root isoperoxidases and isoamylases. Analysis of available nullisomic-tetrasomic lines for chromosomes of group 1 to 7 of the three genomes in different combinations implicated the involvement of IAS, IBS, 6BL, 7AS, 7AL and 7DS in the synthesis of leaf peroxidases. Homeologous chromosomes 6BS, 6DS and 7AL, 7DL are responsible for the synthesis of root isoperoxidase.

Oresent studies, by using CS/rye additional lines revealed that grain isoperoxidases of rye are related to chromosome 1 and 7 whereas root peroxidases showed the presence of rye bands on additional line carrying chromosome 2R and 6R.

The zymogram of grain peroxidase of barley indicated the involvement of chromosome 2H. 5H and 6H in the synthesis of grain peroxidases. As far as root peroxidases are concerned results showed the involvement of 2H and 7H. The localization of genes for leaf and root peroxidases on group 1, 6 and 7 genome of wheat and IR, 6R and 7R of rye an evidence of homaeology between wheat and rye chromosomes.

Zymogram analysis of nullisomic, tetrasmic and ditelosomic aneuploid genotypes of wheat cultivar ‘Chines Spring’ indicated the possible location for the synthesis of leaf isoamylases on 6DS, 7AL and 7DL. Results of root isoamylases demonstrated the involvement of chromosomes IBS, 6BS, 6BL, 6DS and 6DLin the synthesis. The banding profile of CS/rye additional lines suggested the location of genes for leaf amylases on IR, 2R, 3R, 6R and 7P in three different tissues of rye.

The studies on CS/barley addition lines exhibited the involvement of 2H, 7H and 5H chromosomes in the synthesis of grain and root amylases respectively.

Isoperoxidase patterns of flag leaves have been investigated in diploid, terraploid and hexaploid wheats and seventeen species of Aegilops. Most of Aegilops species had been screened previously for resistance against karnal bunt (Tilletia indica). Several were highly resistant to the pathogen. T. aestivum lacks genetic resistance.

The study was further extended to establish biochemical marker(s) characterizing genetic resistance. In all 44 isozymes were determined, many of them were common in wild and cultivated species. Species of market specificity belonged to e. Cylinrica, Ae. ovata, e. biuncialls, Ae. Crassa, e. Juvenalls, Ae. vavilvi and e. triaristata. The present investigation highlights the peroxidase isozyme variation and practical diagnostic usage of isoperoxidases in wheat improvement.

Download Full Thesis
2412.84 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
201.46 KB
2 1 Introduction 1
75.38 KB
  1.1 Electrophoresis 5
  1.2 Isozymes 6
  1.3 Aims And Objectives 8
3 2 Literature Review 9
268.26 KB
  2.1 Classification Of Wheat 9
  2.2 Evolution Of Cultivated Wheat 9
  2.3 Chromosome Complement Of T. Aestivum 11
  2.4 Wheat – Barley Homeology 13
  2.5 Wheat- Rye Homoeology 14
  2.6 Triticale 15
  2.7 Synthetic Wheat 16
  2.8 Amphiploid 18
  2.9 Zymogram Analysis In Triticeae 19
  2.10 Peroxidases 25
  2.11 Amylases 27
  2.12 Isoperoxidases And Isoamylases In Tririceae 28
  2.13 Chromosome Location Of Genes Of Enzymes In Triricease 32
  2.14 Isoperoxidases In Relation To Resistance Against Kernel Bunt ( Tilletia Indica ) In Wild And Cultivated Wheat 35
4 3 Materials And Methods 36
171.56 KB
  3.1 Plant Material 36
  3.2 Biochemical Methods 50
5 4 Results 57
1182.71 KB
  4.1 Isozyme Variation 57
  4.2 Chromosome Location Of Genes 161
  4.3 Isoperoxidases In Relation To Resistance Against Kernel Bunt ( Tilletia Indica ) In Wild And Cultivated Wheats. 199
6 5 Discussion 204
432.97 KB
  5.1 Isozyme Variation 204
  5.2 Chromosome Location Of Genes 230
  5.3 Isoperoxidases In Relation To Resistance Against Kernel But ( Tilletia Indica ) In Wild And Cultivated Wheat 241
7 6 References 245
383.33 KB
8 7 Appendices 282
60.58 KB