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PRIMARY STRUCTURE OF HEMOGLOBIN'S (A) ABNORMAL HUMAN MEMOGLOBINS (B) HEMOGLOBINS FROM JAGAR (PANTHERA ONCO) & LEOPARD (PANTHERA PARDUS)

Ahmed, Aftab (1988) PRIMARY STRUCTURE OF HEMOGLOBIN'S (A) ABNORMAL HUMAN MEMOGLOBINS (B) HEMOGLOBINS FROM JAGAR (PANTHERA ONCO) & LEOPARD (PANTHERA PARDUS). PhD thesis, University of Karachi, Karachi.

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Abstract

The present study on abnormal hemoglobin's, jaguar (panthera onco) and North Persian leopard (panthera pardus) hemoglobins were undertaken to characterize the abnormality in the hemoglobin variants and the primary structure of globin chains respectively. Different electrophoretic methods i.e. cellulose acetate membrance Polyacrylamide gel disc, isoelectric focusing in thin layer IEE gel and Trition-PAG were used to identify the hemoglobin components and the globin chains. Hemoglobin components and globin chains were isolated on DEAE-Sephacel and CM-Cellulose in the presence of dissociating agent 8M urea respectively. Reversed phase HPLC was employed successfully for the isolation of the globin chains and the fingerprinting of tryptic peptides. The complete primary structures of abnormal peptides and of the globin chains were established by using the liquid and gas-phase sequencer. Sequence study on abnormal hemoglobins results in the characterization of three variants. 1).Hb-Ea2b226Glu-Lys inassocation with B-thalassemia disorder. 2).A new hemoglobin vartiant Hb-karachi a2B2. 3).Hb-Andrew-Minneapolis a2B2 144Lys-Asn in a German family (Father and his son) from berlin. The study on animal hemoglobin results in the characterization of 6 globin chains i.e. one alpha, and two B-chains in each animal. The sequence alignment with the members of the family felidae reveals high degree of homology both in the and chains

Item Type:Thesis (PhD)
Uncontrolled Keywords:hemoglobin, memoglobins, jagar (panthera onco), leopard (panthera pardus), globin chains, hemoglobin structure, mammalian hemoglobins, thalassemia
Subjects:Physical Sciences (f) > Chemistry(f2)
ID Code:834
Deposited By:Mr. Muhammad Asif
Deposited On:27 Oct 2006
Last Modified:04 Oct 2007 21:03

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