I= AMINO ACID SEQUENCE OF MAJOR NEUROTOXINS FROM BUTHUS SINDICUS (SCORPION) VENOM
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Title of Thesis
AMINO ACID SEQUENCE OF MAJOR NEUROTOXINS FROM BUTHUS SINDICUS (SCORPION) VENOM

Author(s)
Aine Fazal
Institute/University/Department Details
H.E.J Research institute of Chemistry/ University of Karachi
Session
1988
Subject
Chemistry
Number of Pages
97
Keywords (Extracted from title, table of contents and abstract of thesis)
amino acid, neurotoxins, buthus sindicus, venom, scorpion, poison gland, scorpion-toxins, venom protein, tryptic peptide, amino acid

Abstract
The present study on the venom of Buthus sindicus was undertaken to characterize scorpion venom proteins. The separation of scorpion venom components was carried out by various Chromatographic techniques. The pure proteins/ peptides were subjected to enzymatic degradation and separated on HPLC Vydac C-18 column. The amino acid compositions of proteins/ peptides were determined after hydrolysis.

Scorpion venom were characterization on the basis of homology and biological activity. The major components of the venom consist of a novel peptide, insect toxin and neurotoxin of 28,35 and 65 amino acid residues respectively. These were sequenced by manual DABITC method and gas phase sequencing.

The fatty acids of scorpion body were also determined by GCMS, LD50 were checked on Rana tigrina muscles and, on files (Musca domestically). The results identify the proteins BuS I and BuS II as a member of “four disulfide core proteins” and extend suggestions of relationship with various peptides involved in binding or interacting functions.

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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
62.58 KB
2 1 Introduction 2
176.95 KB
  1.1 Scorpion 3
  1.2 Classification 3
  1.3 Order 4
  1.4 External Feature 4
  1.5 Sensory Organs 6
  1.6 Poison Gland 7
  1.7 Poisoning Symptoms 8
  1.8 Mode Of Action Of Scorpion-Toxins 9
  1.9 Purification Of Scorpion Venom 10
  1.10 Structure Of Venom Protein 12
  1.11 Structure Function Relationship 15
  1.12 Biological Activities Of Scorpion Venom 20
  1.13 Objection Of The Present Studies 21
3 2 Materials And Methods 22
95.52 KB
  2.1 Sample Collection And Identification 23
  2.2 Extraction Of Venom 23
  2.3 Purification Of Proteins 23
  2.4 Electrophoresis 24
  2.5 Modification Of Venom Proteins 28
  2.6 Enzymatic Cleavage 29
  2.7 Tryptic Peptide Separation By Rp-Hplc 29
  2.8 Amino Acid Analysis 29
  2.9 Amino Acid Sequencing Of Proteins And Peptides 30
  2.10 Analysis Of Scorpion Fatty Acids By Gas Chromatography-Mass Spectrometry ( Gcms ) 33
  2.11 Biological Activities Of The Venom 34
4 3 Results 37
178.93 KB
  3.1 Venom 38
  3.2 Electrophoresis 38
  3.3 Separation Of Scorpion Venom Proteins On Rp-Hplc 38
  3.4 Molecular Mass Of The Separated Peaks By Sds-Page 38
  3.5 Characterization Of Peptides 42
  3.6 Biological Activities Of The Venom 64
  3.7 Scorpion Fatty Acid Determination By Gcms 68
5 4 Discussion 72
133.54 KB
  4.1 Characterization Of Novel Short Peptide Bus I 73
  4.2 Identification Of Insect Toxin Like Peptide Bus II 76
  4.3 Characterization Of Neurotoxin Bus Ivb 80
  4.4 Partial Sequence Of Toxin Bus V 82
  4.5 N -Terminal Partial Sequence Of Neurotoxins( Bus VI, Bus VII) 84
  4.6 Biological Activities Of The Venom 86
  4.7 Scorpion Fatty Acid Determination By GCMS 88
6 5 References 89
75.03 KB