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Title of Thesis

Molecular Detection Of Salmonella Typhi Strains & Their Drug Resistance Pattern

Author(s)

Asma Haque

Institute/University/Department Details
National Institute For Biotechnology & Genetic Engineering Quaid-i-Azam University, Islamabad
Session
2005
Subject
Pathology
Number of Pages
178
Keywords (Extracted from title, table of contents and abstract of thesis)
Typhoid, Resistance, Typhi, Drug, Diagnostic, Patterns, Fragment, Transformed, Salmonella, Molecular, Detection, Strains

Abstract
Typhoid fever has become a major problem due to the emergence of MDR strains of Salmonella enterica serovar Typhi, the causative agent, at an alarming rate during recent years.The situation is worsened due to lack of quick, sensitive and reliable diagnostic tools for determining the drug resistance pattern. Conventional methods are time consuming and lack sensitivity.It was envisaged that a multiplex PCR diagnosing typhoid and detecting resistance against standard typhoid drugs, ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole and ciprofloxacin would be very useful.After determining drug resistance patterns by standard disc diffusion method on a pool of 23 MDR S. Typhi isolates, a PCRamplification technique was used for various drug resistance related genes found universally in S. Typhi.These were tem, catP, and sul2 genes. None of these isolates was resistant to ciprofloxacin, so a fragment of gyrA gene (related to ciprofloxacin resistance) was amplified from an MDR E.coli isolate, cloned, and transformed into an MDR S.Typhi isolate that was naturally resistant to other drugs.A regular multiplex PCR was subsequently developed by using this cloned bacterium which was followed by the development of a nested multiplex PCR for increasing specificity and sensitivity.This diagnostic multiplex PCR has been successfully optimized to be directly applicable to clinical samples.

Download Full Thesis
2,568 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 CONTENTS

 

 
181 KB
2

1

INTRODUCTION AND REVIEW OF LITERATURE


 

1
464 KB
3 2 MATERIALS AND METHODS

 

34
291 KB
4 3 RESULTS

 

68
1,519 KB
5 4 DISCUSSION

 

108
197 KB
6 5 REFERENCES AND APPENDICES

116
292 KB