I= STUDIES ON THE FATTY ACID AND OTHER LIPID CONSTITUENTS OF FISH ORIGIN.
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Title of Thesis
STUDIES ON THE FATTY ACID AND OTHER LIPID CONSTITUENTS OF FISH ORIGIN.

Author(s)
Faheem Ahmed
Institute/University/Department Details
University of Karachi, Pakistan. /Department of Pharmaceutical Chemistry
Session
1995
Subject
FISH ORIGIN
Number of Pages
286
Keywords (Extracted from title, table of contents and abstract of thesis)
fatty acid, lipid constituents, fish , fish oil, hyperlipidemia, carcharhinus melanopterus (black shark), lutjanus johnii (hira), scomberoides iysan (aal), parastromateus niger (black poplet), scomberomorus guttatus (surmai), epinephelus malabaricus (gisser)

Abstract
Seven species of marine fish found in coastal waters of Karachi were studied e.g. Carcharhinus melanopterus (black Shark), Lutjanus Johnii (Hira), Scomberoides Iysan (Aal), Parastromateus niger (Black poplet), Scomberomorus guttatus (Surmai) and Epinephelus malabaricus (Gisser) for their fatty acid composition. The isolation, identification and characterization of thses fatty acids were carried out by gas chromatography and gas chromatography-mass spectroscopy (GC-MS). The total lipid contrnts in liver range from 3.56 to 39.55% of wet tissue and flesh contained highly limited quantity as 0.007-0.805%.

As many as 25 individual fatty acids that occur in marine fish oil were studied. It was analysed that among saturated fatty acid myristic, palmitic and stearic acid are the major whereas lauric, pentadecanoic acid as minor constituents. Among unsaturated fatty acid monoenoic e.g palmitoleic and oleic acids are the major unsaturated fatty acids. Traces of dienoic and trienoic fatty acids were noticed. In addition medicinally important polyunsatured. During the course of present investigation composition of fish oils with reference to climatic and seasonal variations were also evaluated and it was concluded that temperature differences between 13 and 23 had little influence on deposition or introversion of polyunsaturated fatty acids.

The fish oil and its constituents have been studied in detail with special reference to cardiac ailments. The discovery of w-3 fatty acids which are abundant in fish, led to a detail investigation about its effect and role as antihypercholesterolemic agent. The effect of fish oil alone and in combination with two other related drugs namely lovastatin and gemifibrozil was found to be a potential cholesterol and triglycerides lowering agent.

Combination therapy is adapted to specify the importance of drug interaction and w-3 fatty acids as a basis of new studies regarding side effects and toxicity. Attempt is also made to decipher the interaction and mechanism of w-3 fatty acids as an antihyperlipidemic agent. It is also suggested that w-3 fatty acid acts in synergistic relation to that of lovastatin.

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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
47.11 KB
2 1 Abstract 1
18.38 KB
3 2 Introduction 3
335.46 KB
  2.1 Fatty Acid Composition of Fish Oil 3
  2.2 Seasonal Variations in Fatty Acids Composition of Fish Oil 6
  2.3 Hyperlipidemia and Its Classification 7
  2.4 Treatment of Hyperlipidemia 10
  2.5 Application of Fish Oil in The Remedy of Various Diseases 16
  2.6 Analytical Tools Applied for the Analysis of Fish Oil 42
  2.7 Aims and Objectives 50
4 3 Experimental Work. 51
133.85 KB
  3.1 Extraction, Isolation and Characterization of Fatty Acid In fish oil. 51
  3.2 Biochemical Studies 57
5 4 Results and Discussion 72
1213.66 KB
  4.1 Analytical Techniques Utilized for Characterization of Fatty 72
  4.2 Biochemical Studies of Fish Oil 132
  4.3 Statistical Analysis 237
6 5 General Discussion 249
50.19 KB
7 6 Conclusions 255
25.77 KB
8 7 Bibliography 258
205.29 KB