NOVEL FORMULATION AND EVALUATION OF MICROEMULSIONS CONTAINING ANTIOXIDANT EXTRACTS OF VARIOUS PLANTS

RASHIDA, PARVEEN (2015) NOVEL FORMULATION AND EVALUATION OF MICROEMULSIONS CONTAINING ANTIOXIDANT EXTRACTS OF VARIOUS PLANTS. Doctoral thesis, The Islamia University of Bahawalpur.

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Abstract

Microemulsion is a more sophisticated form of emulsion to improve the stability and appearance as compared to simple emulsions or macroemulsion. Microemulsion preparations of various cosmetic products are used as moisturizing, sunscreens and smoothing agents. The aim of this study was to develop thermodynamically stable microemulsions with plants extracts, their characterization and in vivo study on volunteers. Extracts of Lycopersicon esculentum (F1), Carica papaya (F2) and Punica granatum (F3) were successfully prepared. The antioxidant activities of the plant extracts were measured by DPPH radical scavenging assay. Standardization of extracts and construction of pseudo phase diagram was done by aqueous titration method. Stable MEs, of F1, F2 and F3 of o/w type were prepared using oily phase (IPM, Palm oil), Tween 80 and propylene glycol as emulsifiers while distilled water was used as aqueous phase. Crude plant extract 10% and 4% was used in F1, F2 and F3, respectively while base (B1 and B2) was utilized without extracts. All these MEs were kept at four different storage conditions, i.e. 8ºC, 25ºC, 40ºC and 50ºC for a period of three months to evaluate their stability characteristics. Human volunteers were used for in vivo evaluation of F1, F2 and F3 to estimate their effects on human skin. Different skin parameters like skin melanin, erythema, skin pH, moisture contents, elasticity, sebum contents and surface evaluation of living skin (SELS) were measured at different time intervals for three months. The results obtained were compared with zero hour readings. Statistical tools of ANOVA and paired sample t-test were used to evaluate the results. Three plants extracts showed significant antioxidant potential. Electrical conductivity results confirmed that an outer phase of MEs was water. Significant (P ? 0.05) differences in the level of melanin were produced by F3. In case of sebum contents, insignificant (P > 0.05) difference was observed for F1, F2 and F3. Significant decrease in erythema was observed in F1, F2 and F3, while F1, F2 and F3 showed insignificant differences in skin pH. A significant (P ? 0.05) increase in elasticity was produced by F1, F2 but F3 showed insignificant differences whereas F1, F2 and F3 showed significant differences on SELS parameters. As a conclusion F1 and F2 MEs with 10% plant extract were found more effective with anti-aging properties. F3 MEs with 4% plant extracts was effective, safe with whitening effect as well as anti-aging properties. Key Words: Lycopersicon esculentum (F1), Carica papaya (F2), Punica granatum (F3), Microemulsions, DPPH, Extracts, Melanin, Sebum, Skin pH, SESL

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: NOVEL,MICROEMULSIONS, ANTIOXIDANT ,EXTRACTS ,PLANTS
Subjects: R Medicine > R Medicine (General)
Depositing User: Mr Sami Uddin
Date Deposited: 16 Oct 2017 10:55
Last Modified: 16 Oct 2017 10:55
URI: http://eprints.hec.gov.pk/id/eprint/6534

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