Pakistan Research Repository

ANALYSIS OF MORPHOLOGICAL IMMUNOHISTOCHEMICAL AND GENETIC PROGNOSTIC DETERMINANTS IN PREDICTING DISEASE FREE SURVIVAL OF BREAST CARCINOMA PATIENTS

Aziz, Syed Abdul (2001) ANALYSIS OF MORPHOLOGICAL IMMUNOHISTOCHEMICAL AND GENETIC PROGNOSTIC DETERMINANTS IN PREDICTING DISEASE FREE SURVIVAL OF BREAST CARCINOMA PATIENTS. PhD thesis, University of Karachi, Karachi.

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Abstract

Breast cancer is an increasingly important cause of illness and death among women worldwide. In Pakistan also breast cancer is not only the commonest malignancy among women but it also occurs in a younger age compared to western population. This prospective study was conducted on 315 consecutive human breast biopsy and mastectomy specimens with lymph node sampling received from 1992 to 1997 with histological diagnosis of infiltrating ductal carcinoma breast. This study also included specimens from high-risk group i.e in-patient with informatory breast carcinoma and with pregnancy/lactation associated breast carcinoma. The major objective of the study was to assess the utility of novel prognostic markers in assessing the outcome of disease in Pakistani breast carcinoma patients. Novel prognostic markers studied included growth promoting genes products i.e C-crbB2 and EGFR. Tumour suppressor gene product i.e p53, proliferative market PCNA, along with a lysosomal protease i.e., Cathepsin-D. Established parameters and prognostic variables of breast cancer like age of the patient, menstrual status, tumour size histological grade, axillary lymph node status (distant metastases if any), Hormones Receptor (estrogen and progesterone) status were studied in parallel to these novel prognostic markers. In this study p53, C-erbB2, EGFR and PCNA showed significant correlation with various established pathological variables as well as with disease free and overall survival. It was shown that p53, CerbB2, EGFR and PCNA were independent prognostic markers in both univariate and multivariate analysis. In our study cathepsin-D over-expression was not a good prognostic marker if only expression in tumour cells was taken into account. In summary as breast cancer becomes a disease, which is now diagnosed and treated at an early stage in the west, these modern tools of investigation in parts of the world where this still presents in an advanced manner can serve as a useful method in understanding the biological behavior of breast cancer. This thesis is an attempt to identify predictive factors in breast cancer important for regional and distant spread as well as for disease free and overall survival in our clinical setting.

Item Type:Thesis (PhD)
Uncontrolled Keywords:morphological immunohistochemical, genetic prognostic determinants, breast carcinoma, breast cancer, tumor, epidermal growth factor receptor, cathepsin-d., proliferating cell nuclear antigen (pcna), inflammatory breast carcinoma (ibc), prefnancy/lactation associated breast carcinoma (pac)
Subjects:Biological & Medical Sciences (c) > Biological Sciences(c1) > Evolution & genetics (c1.6)
ID Code:651
Deposited By:Mr. Muhammad Asif
Deposited On:14 Sep 2006
Last Modified:04 Oct 2007 21:02

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