I=
Pakistan Research Repository Home
 

Title of Thesis

Molecular Characterization and the Potential use of Begomovirus Associated DNA 1 as a Silencing/ Expression Vector

Author(s)

Muhammad Shafiq Shahid

Institute/University/Department Details
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad & Quaid-i-Azam University, Islamabad
Session
2010
Subject
Biotechnology and Genetic Engineering
Number of Pages
214
Keywords (Extracted from title, table of contents and abstract of thesis)
Molecular, Characterization, Potential, Begomovirus, Associated, DNA, Silencing, Expression, Vector

Abstract
Available

Download Full Thesis
4,746 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 CONTENTS

 

v
32 KB
2

1

INTRODUCTION AND REVIEW OF LITERATURE

1.1 Plant viruses
1.2 Geminiviruses
1.3 Genome organization of geminiviruses
1.4 Functions of geminivirus proteins
1.5 Satellites associated with geminiviruses
1.6 Transmission of geminiviruses
1.7 Geminivirus-host interactions
1.8 Movement of geminiviruses
1.9 Impact of begomoviruses
1.10 Recombination among begomoviruses
1.11 Favored features for recombination among geminiviruses
1.12 Replication of geminiviruses
1.13 Natural plant defense against viruses
1.14 Control of plant diseases
1.15 Other approaches for disease resistance
1.16 RNA silencing
1.17 RNA silencing through the exogenous introduction of transgene

1
803 KB
3 2 MATERIALS AND METHODS

2.1 DNA Manipulation
2.2 Amplification of DNA
2.3 Cloning of amplified DNA
2.4 Transformation of competent cells
2.5 DNA Analysis
2.6 Purification of DNA
2.7 Microbiological Techniques

51
292 KB
4 3 AN ALPHASATELLITE IS NO LONGER A CONSTANT FEATURE OF THE COTTON LEAF CURL DISEASE EGOMOVIRUS/BETASATELLITE COMPLEX

3.1 Introduction
3.2 Methodology
3.3 Results
3.4 Discussion

64
926 KB
5 4 INVESTIGATION OF THE USE OF AN ALPHASATELLITE AS A SILENCING AND EXPRESSION VECTOR

4.1 Introduction
4.2 Methodology
4.3 Results
4.4 Discussion

84
477 KB
6 5 EFFECTS OF AN UNUSUAL ALPHASATELLITE ON SYMPTOMS INDUCED BY BEGOMOVIRUSES ORIGINATING FROM OMAN

5.1 Introduction
5.2 Methodology
5.3 Results
5.4 Discussion

103
1,108 KB
7 6 GENERAL DISCUSSION


123
170 KB
8

7

REFERENCES

 

129
355 KB