MOLECULAR SUB-TYPING OF ROTAVIRUSES CAUSING INFECTION IN CHILDREN

Tayyba Iftikhar, . (2015) MOLECULAR SUB-TYPING OF ROTAVIRUSES CAUSING INFECTION IN CHILDREN. Doctoral thesis, Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad.

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Abstract

Diarrheal diseases remain a common cause of morbidity and mortality in young children. Acute gastroenteritis ranks among one of the six main causes of all deaths. It is now considered that rotaviruses are leading cause of gastroenteritis especially in young children ?5 years of age. Due to lack of any surveillance network, Pakistan does not keep any epidemiological record regarding the local reservoirs of rotavirus strains. Many of the rotavirus epidemics and hospitalizations go unobserved and/or never monitored for the rotaviruses or its genotypic/serotypic variants except very few studies during the last decades. The study was conducted to identify G and P genotypes. Sequencing of selected electropherotypes was done for better understanding of phylogenetic analysis, comparison of amino acids sequences and Genbank sequence submission of G and P types. G1P[8] was the most prevalent genotype followed by mixed infection of G1P[4]P[8]. Sequencing and deduced amino acids analysis of VP4, VP7 and NSP4 were found to be closely related to globally circulating rotavirus strains. Comparison between Faisalabad samples and globally circulating strains identified different amino acids substitutions in variable regions at different positions. The current study and baseline data produced will be helpful in decisions about need for rotavirus vaccine programs, knowledge and awareness of the disease among physicians and medical virologists.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: MOLECULAR,ROTAVIRUSES,INFECTION,CHILDREN
Subjects: Q Science > Q Science (General)
Depositing User: Ms Maryam Saeed
Date Deposited: 16 Oct 2017 05:36
Last Modified: 16 Oct 2017 05:36
URI: http://eprints.hec.gov.pk/id/eprint/6426

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