Theoretical Analysis of Dynamic Behaviors in Liquid Chromatography

Javeria Nawaz Abbasi, . (2015) Theoretical Analysis of Dynamic Behaviors in Liquid Chromatography. Doctoral thesis, COMSATS Institute of Information Technology Lahore Campus - Pakistan.

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Abstract

The chromatographic techniques are used on laboratory and industrial scales for the sepa ration of substances that under the traditional processes, such as distillation or extraction, are neither technically nor economically feasible. It is an important separation technique in the petrochemical industry and becomes more and more exploited in ?ne chemical, phar maceutical and biotechnical industries. For instance, this attractive technology is used to separate chiral molecules, enzymes, sugar and to purify proteins or to produce insulin. This thesis project is concerned with the analytical and numerical solutions of three stan dard liquid chromatographic models namely, the equilibrium dispersive model (EDM), the lumped kinetic model (LKM) and the general rate model (GRM). Each model consid ers di?erent levels of complexities to describe the process. These models are systems of convection-di?usion partial di?erential equations with dominating convective terms and coupled through di?erential or algebraic equations. The Laplace transformation is applied to derive the analytical solutions of the EDM and LKM considering the special case of single-component linear adsorption isotherm, contin uous or ?nite width pulse injections, two di?erent sets of boundary conditions and fully porous particles. For further analysis of the solute transport behavior, the analytical tem poral moments are derived from the Laplace-transformed solutions and are compared with the numerical solutions of a semi-discrete high resolution ?nite volume scheme (HR-FVS). For nonlinear adsorption isotherms, numerical techniques are the only tools to provide solu tions. However, the strong nonlinearities of realistic thermodynamic functions pose major di?culties for the numerical schemes. For that reason, computational e?ciency and accu racy of the numerical methods are highly important. The suggested HR-FVS is extended to approximate these nonlinear model equations. The numerical results of the suggested HR-FVS are compared with some other ?nite volume schemes available in the literature. Di?erent case studies are considered covering a wide range of mass transfer kinetics. The results obtained veri?ed the correctness of analytical results and accuracy of the suggested HR-FVS. An interesting aspect of this thesis project is the application of GRM to ?xed-bed chro matographic columns packed with core-shell or fully porous particles. Due to their proven performance and improved availability, core-shell particles are increasingly applied for chro matographic separations. Such particles are useful for highly e?cient and fast separation of complex samples with a reasonably low back pressure. Cored beads provide advantages over fully porous beads, such as reduced di?usional mass transfer resistances in particle macropores and separation times. The concept has improved column e?ciency by shorten ing the di?usion path that molecules have to travel and thus, has improved the di?usional mass transfer kinetics in particle macropores. Once again, both single-component linear and multi-component nonlinear GRM models are considered. The above mentioned ana lytical and numerical solution techniques are applied to solve the model equations. The potential of the solutions is demonstrated by considering di?erent case studies that quan tify the e?ects of the relative core size, axial dispersion, ?lm mass transfer resistance and intraparticle di?usion resistance in the porous layer on the elution curves.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: Theoretical, Dynamic Behaviors , Chromatography
Subjects: Q Science > QA Mathematics
Depositing User: Unnamed user with email jmemon@hec.gov.pk
Date Deposited: 25 Sep 2017 06:48
Last Modified: 25 Sep 2017 06:48
URI: http://eprints.hec.gov.pk/id/eprint/6391

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