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Title of Thesis

Expression of two insecticidal genes in cotton

Author(s)

Allah Bakhsh

Institute/University/Department Details
National Centre Of Excellence In Molecular Biology / University Of The Punjab, Lahore
Session
2010
Subject
Molecular Biology
Number of Pages
172
Keywords (Extracted from title, table of contents and abstract of thesis)
Contribution, Genes, Transgenic, Cotton, Insecticidal, Expression, Two, Plan, Sustainable, Insects, Potential, Fixation

Abstract
Not Available

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2,382 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 CONTENTS

 

xi
35 KB
2

1

INTRODUCTION

 

1
189 KB
3 2 REVIEW OF LITERATURE

2.1 Insect Resistance Management (Two Genes Strategy)
2.2 Temporal and Spatial Expression in Insecticidal Protein
2.3 Factors contributing to endotoxin variability
2.4 RuBisCo gene as Promoter

7
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4 3 MATERIALS AND METHODS

3.1 Experimental Material 39
3.2 Confirmation of Gene Integration and Expression
3.3 Leaf Biotoxicity Assay
3.4 Isolation of RbcS promoter from Gossypium arboretum
3.5 TA Cloning and Transformation of Eluted DNA
3.6 Cloning of RbcS and Cry1Ac in pCAMBIA 1301
3.7 Electroporation of plasmid in Agrobacterium
3.8 Transformation of Gossypium hirsutum Var. 846
3.9 Molecular Analysis of Transgenic Plants (T0 Progeny)
3.10 Molecular Analysis of T1 Progeny

39
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5

4

RESULT

4.1 Confirmation of gene integration and Expression|
4.2 Temporal and Spatial Expression of Cry1Ac and Cry2A
4.3 Leaf Biotoxicity Assay
4.4 Isolation of RbcS promoter from Gossypium arboreum
4.5 Cloning of insecticidal gene under RbcS in pCAMBIA 1301
4.6 Electroporation and Agrobacterium mediated Transformation
4.7 Molecular Analysis of Transgenic Plants (T0 progeny)
4.8 Molecular Analysis of Transgenic Plants (T1 progeny)

70
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6

5

DISCUSSION

 

103
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7

6

LITERATURE CITED AND APPENDICES

 

115
303 KB