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Title of Thesis

Identification and Characterization of Drought Tolerant Genes in Cotton by Gene Homology

Author(s)

Uzma Saeed

Institute/University/Department Details
National Centre Of Excellence In Molecular Biology / University Of The Punjab, Lahore
Session
2009
Subject
Molecular Biology
Number of Pages
203
Keywords (Extracted from title, table of contents and abstract of thesis)
Characterization, Drought, Protect, Independent, Homology, Identification, Stress, Material, Purification, Tolerant, Gene, Cotton

Abstract
Not Available

Download Full Thesis
4,701 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 CONTENTS

 

 
9 KB
2

1

INTRODUCTION

 

1
351 KB
3 2 LITERATURE REVIEW

2.1 Abiotic Stress and its Effect on Plant Growth
2.2 Role of Different Osmoprotectants to Protect Plants against Drought
2.3 Various Regulatory Mechanisms to Confer Stress Tolerance in Plants
2.4 Differential Display

4
572 KB
4 3 MATERIALS AND METHODS

3.1 Plant Material and Stress Treatment
3.2 Total RNA Extraction
3.3 Formaldehyde/ Agarose Gel Electrophoresis
3.4 Quantification of Total RNA
3.5 cDNA Synthesis
3.6 Differential Display PCR
3.7 Agarose Gel Electrophoresis
3.8 Extraction and purification of DNA from the gel
3.9 Reamplification of Eluted Bands
3.10 Sequencing PCR
3.11 Precipitation of Amplified Fragments
3.12 Sequencing of Fragments
3.13 Sequence Alignment

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314 KB
5 4 RESULTS

4.1 RNA extraction and quantification
4.2 cDNA Synthesis
4.3 Amplification of Transcripts
4.4 Sequencing of Transcripts
4.5 Protein Motifs of Transcripts

31
3,482 KB
6 5 DISCUSSION

 

44
184 KB
7

6

REFERENCES AND APPENDICES

 

46
191 KB