Title of Thesis

Gene expression profiling of HCV- associated liver diseases

Author(s)

Dr. Fouzia T. Javed

Abstract
Not Available

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2.1 What is Hepatitis C?
2.2 Types of Hepatitis C
2.3 Hepatitis C virus
2.4 HCV Epidemiology
2.5 Modes of Transmission
2.6 Natural History of Hepatitis C Virus Infections
2.7 HCV Genome and Life Cycle
2.8 Liver response
2.9 Natural History and Diagnosis
2.10 Pathogenesis of Liver Fibrosis
2.11 Microarray

3.1 Study/ Sampling Area
3.2 Inclusion Criteria
3.3 Exclusion Criteria
3.4 Histological evaluation of biopsy samples
3.5 HCV detection and genotyping
3.6 RNA Extraction, Purification and Quality Checking
3.7 Agarose Gel Electrophoresis
3.8 cDNA synthesis (from Isolated RNA) and labeling
3.9 cDNA purification
3.10 aRNA Synthesis and purification
3.11 Labeling with Cy-3 and Cy-5
3.12 Preparation of Cy-3 and Cy-5 monoreactive Dyes
3.13 Post Dye-coupling aRNA purification
3.14 Micro array hybridization
3.15 Scanning of slides
3.16 Data analysis
3.17 Data normalization and analysis
3.18 Validation studies
3.19 Selective cellular gene expression analysis in different stages of fibrosis in HCV-affected liver cells by real-time PCR

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4.1 Histological evaluation
4.2 Genotyping
4.3 Univariate analysis
4.4 Multivariate analysis
4.5 Correlation of biochemical markers with liver fibrosis stages
4.6 RNA isolation from tissue and serum
4.7 Microarray results
4.8 Significant Analysis of Microarray (SAM)
4.9 Cluster analysis of selected genes
4.10 EASE analysis for genes
4.11 Expression Details for different gene Clusters during Fibrosis
4.12 Real-Time PCR analysis of selected genes
4.13 Down-Regulation of Cellular Genes in HCV Patients with Advanced Stages of Fibrosis
4.14 Up-Regulation of Cellular Genes in HCV Patients with Advanced Stages of Fibrosis

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