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PRODUCTION OF PHYTOTOXING FROM ASCOCHYTA RUBIEI, AND MOLECULAR STUDIES OF DEFENCE GENES IN BLIGHT INFECTED CHICKPEA

Shahid, Ahmad Ali (2004) PRODUCTION OF PHYTOTOXING FROM ASCOCHYTA RUBIEI, AND MOLECULAR STUDIES OF DEFENCE GENES IN BLIGHT INFECTED CHICKPEA. PhD thesis, University of the Punjab, Lahore.

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Abstract

The study presented in this thesis is aimed to investigate the production of phytotoxic compounds from Ascochyta rabiei and molecular studies of defence genes in blight infected chickpea. Blight, caused by A.rabiei is one of the most serious disease of chickpea in the world including Pakistan. When a.rabiei was grown on Czapek Dox broth by surface culture method supplemented with an aqueous extract of chickpea seeds, it produced at least three phytotxic compounds, identified as solanapyrone A,B and C. The addition of divalent metal cations such as Zn2+, Ca2+, Mn2+ and Cu2+ to Czapek Dox broth stimulated the production of solanapyrones A,B and C. These phytotoxic compounds were identical to the phytotoxins isolated previously from culture filtrates of the fungus altenaria solani. The compounds were separated by HPLC iwht a column of ODS silica as stationary phase and aqueous mixture of methanol (MeOH), acetonitrile (ACN) and terahydrofuran (THF) as mobile phase. Eluent form the column was monitored by a diode array detector operating in the UV mode.

Item Type:Thesis (PhD)
Uncontrolled Keywords:chickpea, fungal blight, antifungal proteins, toxin, phytotoxic compounds, a.rabiei, antibodies, immunological studies
Subjects:Biological & Medical Sciences (c) > Biological Sciences(c1) > Biology (c13)
ID Code:457
Deposited By:Mr. Muhammad Asif
Deposited On:11 Aug 2006
Last Modified:04 Oct 2007 21:01

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