extracts from four different plant species [Salvia nubicola B.(Laminiaceae), Hedera nepalensis K. (Araliaceae) Acer oblongifolium
D. (Aceraceae)and Sorbaria tomentosa L. (Rosaceae)] were evaluated for their
antimicrobial activity (by
antibacterial and antifungal assays), toxicity activities (by brine
assay, radish seed phytotoxicity assay), antitumor activity (by
potato disc assay) and
antioxidant activities (by DPPH scavenging assay, ABTS+ assay, DNA
Leaf and stem extract of A. oblongifolium exhibited significant
against all pathogenic strains tested, while none of the extract
presented any antifungal
activity against six pathogenic strains tested. Two of the five
extracts (L+S) A.
oblongifolium and (L+S) H. nepalensis revealed significant ED50
value i.e. 47.7 ppm and
226.8 ppm respectively in case of brine shrimp cytotoxicity assay.
Growth inhibition was
observed by all extracts in radish seed bioassay at high
concentration (10,000 ppm). At
low concentration (1000 ppm) three extracts from two plant species
(leaves and flowerextract of S. nubicola, stem extract of S. nubicola and stem extract
of H. nepalensis)
presented stimulation of growth ranging from 3.5 to 43.2%.
Inhibition of tumor formation
ranged from 9 to 82.9% by all extracts in antitumor potato disc
assay at three different
concentrations tested (1000, 100, and 10 ppm). A positive
correlation was observed in the
results of three of the described assays (toxicity assays i.e. brine
assay and phytotoxicity assay and antitumor potato disc assay).
Four methanol extracts from three selected plant species i.e. Salvia
Acer oblongifoium (Aceraceae) and Hedera nepalensis (Araliaceae))
were screened for
their antioxidant potential. Antioxidant activities were
investigated in aqueous system by
using DPPH scavenging assay, ABTS+ radical scavenging assay and DNA
assay while in lipid system by using TBARS (Thiobarbituric acid
Total phenolic contents were determined by using Folin-Ciocalteu
extract of leaf and flower of S. nubicola showed the highest trolox
equivalent values in
case of DPPH scavenging assay i.e. 2484.08 ± 4.9 as well as total
phenolic contents i.e.
342.08 ± 19.8. Fractionation of methanol extract of S. nubicola by
HPLC yielded three fractions (A, B and C). Fraction B was found to
be the most active in
DPPH scavenging assay with highest phenolic contents as estimated by
using Folin-Ciocalteu reagent. Analytical scale HPLC and LC-MS results revealed
rosmarinic acid in fraction B of S. nubicola while chlorogenic acid
and rutin were
identified as major antioxidants in methanol extract of H.