EFFECT OF ORGANIC AND INORGANIC NUTRIENTS AND HORMONES ON GROWTH AND DEVELOPMENT OF TISSUE EXPLANT FROM LOCAL PISTACHIO CULTIVARS CULTURED IN VITRO

Shaista, Jabeen (1993) EFFECT OF ORGANIC AND INORGANIC NUTRIENTS AND HORMONES ON GROWTH AND DEVELOPMENT OF TISSUE EXPLANT FROM LOCAL PISTACHIO CULTIVARS CULTURED IN VITRO. Doctoral thesis, University of the Punjab.

[img] Text
1475.HTM

Download (15kB)

Abstract

Procedures have been developed for the plant regeneration through callus culture. Seeds of Pistacia vera Linn. (pistachio) producing aseptic seedlings were effectively sterilized by dipping in 0.1% ( w Iv ) mercuric chloride solution containing few drops of Tween-20 for 15 minutes. Explants were obtained from aseptically grown seedlings. The cotyledons with intact seed-coats had a beneficial effect on proper sterilization as well as germination rate. Chilling of the seeds at 4 °C for 48 h given before sterilization was found helpful in breaking down. dormancy which in turn improved the rate of germination. MS medium in the semi-solid form was found to be superior to all other salt mixtures in terms of seed survival and germination rate. Cotyledonary explants in the presence of 2.0 mg/L each of 2,4-D and KIN and 3% sucrose produced maximum amount of callus with desired characteristics. NH4CI and L-glutamine could not improve callus growth. Reduction of macro-nutrients of full strength MS medium to ½ or ¼ strength showed no beneficial effect on growth of cotyledonary calli. Reduction in vitamin contents of MS medium to ½ improved calli characteristics (colour and texture) and growth. But reduction of Fe-EDTA to half or zero strength had deterious effect on P. vera L. callus. Addition of 2.0 mg/L of 2,4-D and KIN each in the medium was the most suitable combination of growth regulators for callogenesis of cotyledonary explants. Addition of 10% (v/v) coconut milk delayed browning of the calli and improved growth of healthy calli. Morphogenesis was induced in calli grown on medium containing NAA (0.25 mg/L) and BA (2.0 mg/L).Development of regenerated shoot buds into plantlets was achieved on medium containing NAA (0.25 mg/L), BA (2.0 mg/L) and L-glutamine (250 mg/L). from cotyledonary calli Shoot proliferation of the regenerated buds was achieved on medium containing 2.0 mg/L of KIN and 4.0 mg/L of BA.-GA3 could not improve the growth of regenerated shoots. Rhizogenesis of regenerated shoots was not achieved.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: tissue explant, pistachio, pistacia vera linn, plant regeneration, cotyledons
Subjects: Q Science > QD Chemistry
Depositing User: Muhammad Khan Khan
Date Deposited: 26 Oct 2016 05:08
Last Modified: 26 Oct 2016 05:08
URI: http://eprints.hec.gov.pk/id/eprint/2745

Actions (login required)

View Item View Item