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| Pakistan Research Repository | Home |
Title of Thesis |
| Author(s) SYED ASFAR JAMAL |
| Institute/University/Department Details Department of Microbiology/ University of Karachi |
| Session 2007 |
| Subject Microbiology |
| Number of Pages 68 |
| Keywords (Extracted from title, table of contents and abstract of thesis) pathogenic microorganisms, rickettsia prowazekll, staphylococcus aureus, escherichia coli, nosocomial infections, community acquired infections, enterobacterial repetitive intergenic consensus, methicillin resistance |
Abstract In this study 94 clinical isolates of Staphylococcus were collected from 72 patients. MIC of oxacillin and other important antibiotics were determined.48 of 94 isolates were MRSA, 28 were MSSA and 18 were methicillin resistant coagulase negative Staphylococcus. PCR based molecular typing of coagulase gene was done by restriction enzymes (RFLP analysis). We found eight different patterns in our study; five of them were novel PCR patterns that were not reported earlier. Out of 48 MRSA 25 (52.08%) are of same type indicating a nosocomial origin. The genes for 20 tRNA species in Rickettsia prowazekii have been cloned and sequenced. Two tRNA genes (thrT and tyrU) are located upstream of single tuf gene. The others exist as single transcription units. The genes for EF- TU, ribosomal protein operon S-10, IpxD, fabZ and IpxA were also sequenced. Escherichia coli is one of the major causes of human infections particularly urinary tract infection. In this study, 179 isolates were collected from different sources and their susceptibility pattern was studied. Highest resistance 89.9% was found against ampicillin whereas only 3.9% of isolate showed resistance to imipenam. 40.8% were also extended spectrum beta-Iactamase (ESBL) producers. Representative strains were also typed by enterobacterial repetitive intergenic consensus (ERIC) PCR. However due to variation in banding patterns ERIC-PCR was not found suitable for genotyping large number of E.coli isolates. |
| Download Full Thesis |  2543.37 KB |
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| S. No. | Chapter | Title of the Chapters | Page | Size (KB) |
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| 1 | 0 | Contents | 203.63 KB |
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| 2 | 1 | Introduction | 1 | 305.91 KB |
| 1.1 | General Introduction | 1 | ||
| 1.2 | Staphylococcal Components And Products | 2 | ||
| 1.3 | Genetic Regulation Of Virulence Determinant Expression | 10 | ||
| 1.4 | Epidemiology Of Staphylococcal Disease | 10 | ||
| 1.5 | Treatment Of Staphylococcus Aureus Infections | 17 | ||
| 1.6 | Prevention Of Staphylococcal Diseases | 18 | ||
| 1.7 | Antibiotic Resistance | 19 | ||
| 1.8 | Molecular Mechanisms Of Methicillin Resistance | 23 | ||
| 1.9 | Detection Of Methicillin Resistance In Staphylococcus Aureus And Other Species | 25 | ||
| 1.10 | Typing | 30 | ||
| 1.11 | Significance Of Work | 37 | ||
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| 3 | 2 | Material And Methods | 38 | 60.79 KB |
| 2.1 | Bacterial Isolates | 38 | ||
| 2.2 | Staphylococcus Aureus Identification Tests | 38 | ||
| 2.3 | Detection Of Methicillin Resistance Staphylococcus Aureus (MRSA) And Methicillin Resistance Coagulase Negative Staphylococcus (MRCN) Species. | 39 | ||
| 2.4 | Plasmid Profiling | 40 | ||
| 2.5 | Beta- Iactamase Production | 40 | ||
| 2.6 | Preparation Of Bacterial Iysates | 40 | ||
| 2.7 | PCR Amplification Of Coa And Meca Genes | 41 | ||
| 2.8 | PCR Amplification Of RFLP Of Coagulase Gene | 42 | ||
| 2.9 | MIC Of Different Antibiotics | 42 | ||
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| 4 | 3 | Results | 43 | 319.46 KB |
| 3.1 | Identification Of Staphylococcus Species | 43 | ||
| 3.2 | Detection Of MRSA | 43 | ||
| 3.3 | Production Of B- Iactomase | 44 | ||
| 3.4 | Hemolysis | 44 | ||
| 3.5 | Amplification Of Coa And Meca Gene Products | 44 | ||
| 3.6 | Antibiogram | 46 | ||
| 3.7 | RFLP Analysis Of Coagulase Gene | 48 | ||
| 3.8 | Plasmid Analysis | 55 | ||
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| 5 | 4 | Discussion | 56 | 98.93 KB |
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| 6 | 5 | References | 68 | 1847.18 KB |
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