I= MUTAGENESIS OF ASPERGILLUS NIGER FOR HYPERPRODUCTION OF GLUCOSE OXIDASE TO PREPARE GLUCOSE DIAGNOSTIC KIT
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Title of Thesis
MUTAGENESIS OF ASPERGILLUS NIGER FOR HYPERPRODUCTION OF GLUCOSE OXIDASE TO PREPARE GLUCOSE DIAGNOSTIC KIT

Author(s)
MUHAMMAD ANJUM ZIA
Institute/University/Department Details
Department of Chemistry/ University of Agriculture, Faisalabad
Session
2007
Subject
Chemistry
Number of Pages
154
Keywords (Extracted from title, table of contents and abstract of thesis)
mutagenesis, aspergillus niger, glucose oxidase, glucose diagnostic kit, fungi

Abstract
No Abstract

Download Full Thesis
1930.44 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
146.53 KB
2 1 Introduction 1
90.79 KB
3 2 Review Of Literature 8
379.14 KB
  2.1 Enzyme Production By Fungi 8
  2.2 Glucose Oxidase 8
  2.3 Glucose Diagnostic Kit 34
4 3 Materials And Methods 38
206.12 KB
  3.1 Chemicals And Enzyme 38
  3.2 Microorganism 38
  3.3 Strain Improvement Techniques 39
  3.4 Selection Of Mutant 41
  3.5 Production Of Glucose Oxidase 42
  3.6 Enzyme Assay 44
  3.7 Biomass Estimation 45
  3.8 Carbohydrate Analysis 45
  3.9 Estimation Of Protein Contents 46
  3.10 Determination Of Growth Kinetic Parameters 46
  3.11 Purification Of Glucose Oxidase 48
  3.12 Electrophoresis 50
  3.13 Molecular Mass Determination 53
  3.14 Kinetic And Thermodynamic Studies 54
  3.15 Determination Of Stability 55
  3.16 Glucose Estimation Kit 55
5 4 Results And Discussion 58
1085.65 KB
  4.1 Kill Curve Determination 59
  4.2 Selection And Evaluation Of Mutant 61
  4.3 Production Of Enzyme In Shake Flask 73
  4.4 Purification Of Glucose Oxidase 108
  4.5 SDS-PAGE 115
  4.6 Molecular Mass Determination 115
  4.7 Kinetic And Thermodynamic Studies 116
  4.8 Determination Of Stability 130
  4.9 Glucose Estimation Kit 130
  4.10 Conclusions 138
6 5 Summary 139
48.18 KB
7 6 Literature Cited 142
172.17 KB