I= PURIFICATION AND CHARATERIZATION OF BIOACTIVE PEPTIDES/ PROTEINS FROM MEDICINAL PLANTS
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Title of Thesis
PURIFICATION AND CHARATERIZATION OF BIOACTIVE PEPTIDES/ PROTEINS FROM MEDICINAL PLANTS

Author(s)
MUHAMMAD SHAHID
Institute/University/Department Details
Department of Chemistry/ University of Agriculture, Faisalabad
Session
2007
Subject
Biochemistry
Number of Pages
130
Keywords (Extracted from title, table of contents and abstract of thesis)
bioactive peptides, medicinal plants, hygrophila auriculata, abrus vrecatorius, moringa oleifera, croton tiglium, withania somnifera, antimicrobial peptides, microtermes obesi, odontotermes obesus

Abstract
No Abstract

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2339.3 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
369.46 KB
2 1 Introduction 1
42.85 KB
3 2 Review Of Literature 4
351.73 KB
  2.1 Hygrophila Auriculata 4
  2.2 Abrus Vrecatorius 5
  2.3 Moringa Oleifera 7
  2.4 Croton Tiglium 10
  2.5 Withania Somnifera 11
  2.6 Antimicrobial Peptides/ Proteins From Plants Source 14
4 3 Materials And Methods 25
1214.54 KB
  3.1 Extraction Of Antimicrobial Proteins/ Peptides From Leaves And Seeds Of The Selected Medicinal Plants 27
  3.2 Analysis Of Biochemical Parameters Of The Medicinal Plants Under Study 27
  3.3 Antitermitic Activity Against Microtermes Obesi Of The Plant Extracts And Effect On Behavior And Physiology Of Odontotermes Obesus 29
  3.4 Ames Test Of The Samples For The Carcinogenicity 31
  3.5 Coagulation Assay Of The Crude Protein For The Water Treatment 31
  3.6 Antibacterial Assay Of Crude And Partially Purified Extracts 32
  3.7 Antifungal Assay Of Crude And Partially Purified Extracts. 33
  3.8 Minimum Inhibitory Concentration (MIc) Of Plant Extracts On Fungal And Bacterial Strains 34
  3.9 Treatment Of The Crude Extracts With Proteinase K, Trypsin And Heat 34
  3.10 Purification By Ammonium Precipitation And Column Chromatographic Techniques 35
  3.11 Characterization By Optimization Of Ph, Temperature And Ionic Concentration 38
  3.12 Polyacrylamide Gel Electrophoresis And Molecular Mass, Subunit Study 38
5 4 Results And Discussion 40
1214.5 KB
  4.1 Extraction Of The Proteins/Pep Tides From Leaves And Seeds Of The Selected Medicinal Plants. 40
  4.2 Analysis Of Biochemical Parameters Of The Medicinal Plants Under Studv. 42
  4.3 Antitermitic Activity Of The Selected Medicinal Plants (Seeds And Leaf Extracts). 52
  4.4 Ames Test Of The Samples For Carcinogenicity 60
  4.5 Coagulation Assay Of The Crude Protein Extracts From Moringa Olei(Era Water Treatment. 62
  4.6 Antibacterial/Antifungal Activity Of The Medicinal Plants 70
  4.7 Minimum Inhibitory Concentration (MIC) Of Extracts Against Fungal And Bacterial Strains. 77
  4.8 Antibacterial And Antifungal Activities After Treatment Of Crude Extracts With Proteinase K, Trypsin And Heat. 79
  4.9 Partial Purification 81
  4.10 Characterization Of The Protein Extract Of Selected Medicinal Plant At Different Ph And Ionic Concentrations 84
  4.11 Purification Of Antimicrobial Protein/Peptide From Moringa Olei (Era And Croton Tilium. 88
  4.12 Conclusion 101
  4.12 Extension Of The Project And Future Prospects 101
6 5 Summary 102
464.67 KB
  5.1 Literature Cited 104