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| Pakistan Research Repository | Home |
Title of Thesis |
| Author(s) MUHAMMAD SHAHID |
| Institute/University/Department Details Department of Chemistry/ University of Agriculture, Faisalabad |
| Session 2007 |
| Subject Biochemistry |
| Number of Pages 130 |
| Keywords (Extracted from title, table of contents and abstract of thesis) bioactive peptides, medicinal plants, hygrophila auriculata, abrus vrecatorius, moringa oleifera, croton tiglium, withania somnifera, antimicrobial peptides, microtermes obesi, odontotermes obesus |
Abstract |
| Download Full Thesis |  2339.3 KB |
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| S. No. | Chapter | Title of the Chapters | Page | Size (KB) |
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| 1 | 0 | Contents | 369.46 KB |
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| 2 | 1 | Introduction | 1 | 42.85 KB |
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| 3 | 2 | Review Of Literature | 4 | 351.73 KB |
| 2.1 | Hygrophila Auriculata | 4 | ||
| 2.2 | Abrus Vrecatorius | 5 | ||
| 2.3 | Moringa Oleifera | 7 | ||
| 2.4 | Croton Tiglium | 10 | ||
| 2.5 | Withania Somnifera | 11 | ||
| 2.6 | Antimicrobial Peptides/ Proteins From Plants Source | 14 | ||
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| 4 | 3 | Materials And Methods | 25 | 1214.54 KB |
| 3.1 | Extraction Of Antimicrobial Proteins/ Peptides From Leaves And Seeds Of The Selected Medicinal Plants | 27 | ||
| 3.2 | Analysis Of Biochemical Parameters Of The Medicinal Plants Under Study | 27 | ||
| 3.3 | Antitermitic Activity Against Microtermes Obesi Of The Plant Extracts And Effect On Behavior And Physiology Of Odontotermes Obesus | 29 | ||
| 3.4 | Ames Test Of The Samples For The Carcinogenicity | 31 | ||
| 3.5 | Coagulation Assay Of The Crude Protein For The Water Treatment | 31 | ||
| 3.6 | Antibacterial Assay Of Crude And Partially Purified Extracts | 32 | ||
| 3.7 | Antifungal Assay Of Crude And Partially Purified Extracts. | 33 | ||
| 3.8 | Minimum Inhibitory Concentration (MIc) Of Plant Extracts On Fungal And Bacterial Strains | 34 | ||
| 3.9 | Treatment Of The Crude Extracts With Proteinase K, Trypsin And Heat | 34 | ||
| 3.10 | Purification By Ammonium Precipitation And Column Chromatographic Techniques | 35 | ||
| 3.11 | Characterization By Optimization Of Ph, Temperature And Ionic Concentration | 38 | ||
| 3.12 | Polyacrylamide Gel Electrophoresis And Molecular Mass, Subunit Study | 38 | ||
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| 5 | 4 | Results And Discussion | 40 | 1214.5 KB |
| 4.1 | Extraction Of The Proteins/Pep Tides From Leaves And Seeds Of The Selected Medicinal Plants. | 40 | ||
| 4.2 | Analysis Of Biochemical Parameters Of The Medicinal Plants Under Studv. | 42 | ||
| 4.3 | Antitermitic Activity Of The Selected Medicinal Plants (Seeds And Leaf Extracts). | 52 | ||
| 4.4 | Ames Test Of The Samples For Carcinogenicity | 60 | ||
| 4.5 | Coagulation Assay Of The Crude Protein Extracts From Moringa Olei(Era Water Treatment. | 62 | ||
| 4.6 | Antibacterial/Antifungal Activity Of The Medicinal Plants | 70 | ||