I= CHARACTERIZATION OF VENOM PROTEINS FROM TWO SPECIES OF SCORPIONFISH GROUP; HYPODYTES RUBRIPINNIS & SYNANCEJA VERRUCOSA
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Title of Thesis
CHARACTERIZATION OF VENOM PROTEINS FROM TWO SPECIES OF SCORPIONFISH GROUP; HYPODYTES RUBRIPINNIS & SYNANCEJA VERRUCOSA

Author(s)
AMBREENA SIDDIQ
Institute/University/Department Details
International Centre for Chemical Sciences/ HEJ Research Institute of Chemistry/ University of Karachi
Session
2002
Subject
Chemistry
Number of Pages
106
Keywords (Extracted from title, table of contents and abstract of thesis)
venom proteins, hypodytes rubripinnis, synanceja verrucosa, redfin velvetfish, mitogenic activities, cytotoxic activities, mitogenic toxins

Abstract
This study describes the effect of venom from two species of scorpionfish; Hypodytes rubripinnis and Synanceja verrucosa on normal and malignant cell lines with especial reference to protein components, which may cause selective proliferation and/or lysis of the cell lines and to monitor the extent of such effects. Flow cytometric analysis also enabled us to follow the effect on morphology of individual cells. We have attempted to characterize the two venoms in terms of their biological properties, and the findings reported here indicate that venom from both species possess mitogenic as well as cytotoxic properties with differentiation of cell lines.

Hypodytes rubripinnis, commonly known as 'redfin velvetfish' is amongst the dangerous species of scorpion-fish that elicit severe effects on humans following envenomation including intense pain, swelling around the area of sting and tissue necrosis.

S. verrucosa commonly known as stonefish is a well-known specie of the scorpion-fish group. Venom from S. verrucosa has been studied extensively for its pharmacological and enzymatic properties.

The first part of the present study is based on the comparison of some biological activities of venom from the two species. Venom from H. rubripinnis caused agglutination of rabbit erythrocytes, and showed mitogenic and cytotoxic activities on different cell lines. Chromatography of crude venom was performed using gel filtration. The fractions obtained were analyzed for agglutination, mitogenesis and cytotoxicity. Fraction II obtained after gel filtration of crude venom showed cytotoxic activity and was further fractionated on reversed phase HPLC. In the present study we have isolated a 90-kDa protein using gel filtration and reversed phase HPLC, possessing cytotoxic activity. Further studies are required to characterize this protein.

Crude venom from S. verrucosa was also subjected to mitogenic, cytotoxic and flow cytometric analysis, using different cell lines. The venom proteins were fractionated on Sephadex G-200 and RP-HPLC. Two low molecular mass proteins were found to be homogenous on SDS PAGE. Amino acid analysis of these proteins revealed close resemblance to lectins isolated from marine venoms. Further studies are required to characterize the biological activities of these fractions.

Download Full Thesis
1051.93 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
128.05 KB
2 1 Introduction
338.03 KB
  1.1 Venomous Fish 2
  1.2 Biochemical Studies On Venoms 3
  1.3 Cytotoxicity Of Venoms 4
  1.4 Mitogenic Toxins 8
  1.5 Pharmacological Activities 10
  1.6 Enzymatic Activities 12
  1.7 Hemolytic Activities 12
  1.8 Scorpionfish 13
  1.9 Description Of Hypodytes Rubripinnis 18
  1.10 Description Of Synanceja Verrucosa 19
  1.11 Objective Of The Current Study 21
3 2 Materials And Methods
85.66 KB
  2.1 Collection Of Samples 23
  2.2 Extraction Of Venom 23
  2.3 Protein Estimation 23
  2.4 Electrophoresis 24
  2.5 Gel Filtration 24
  2.6 High Performance Liquid Chromatography 25
  2.7 Amino Acid Analysis 25
  2.8 Agglutinating Activity 26
  2.9 Mitogenic Activity 26
  2.10 Cytotoxic Activity 28
  2.11 Cell Flow Cytometry 29
4 3 Results
352.09 KB
  3.1 Characterization Of Hypodytes Rubripinnis Venom 33
  3.2 Characterization Of Synanceja Verrucosa Venom 56
5 4 Discussion
111.03 KB
  4.1 Hypodytes Rubripinnis 74
  4.2 Synanceja Verrucosa 81
6 5 Conclusions And Future Aspects 87
18.37 KB
7 6 References 89
174.69 KB