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| Pakistan Research Repository | Home |
Title of Thesis |
| Author(s) AMBREENA SIDDIQ |
| Institute/University/Department Details International Centre for Chemical Sciences/ HEJ Research Institute of Chemistry/ University of Karachi |
| Session 2002 |
| Subject Chemistry |
| Number of Pages 106 |
| Keywords (Extracted from title, table of contents and abstract of thesis) venom proteins, hypodytes rubripinnis, synanceja verrucosa, redfin velvetfish, mitogenic activities, cytotoxic activities, mitogenic toxins |
Abstract Hypodytes rubripinnis, commonly known as 'redfin velvetfish' is amongst the dangerous species of scorpion-fish that elicit severe effects on humans following envenomation including intense pain, swelling around the area of sting and tissue necrosis. S. verrucosa commonly known as stonefish is a well-known specie of the scorpion-fish group. Venom from S. verrucosa has been studied extensively for its pharmacological and enzymatic properties. The first part of the present study is based on the comparison of some biological activities of venom from the two species. Venom from H. rubripinnis caused agglutination of rabbit erythrocytes, and showed mitogenic and cytotoxic activities on different cell lines. Chromatography of crude venom was performed using gel filtration. The fractions obtained were analyzed for agglutination, mitogenesis and cytotoxicity. Fraction II obtained after gel filtration of crude venom showed cytotoxic activity and was further fractionated on reversed phase HPLC. In the present study we have isolated a 90-kDa protein using gel filtration and reversed phase HPLC, possessing cytotoxic activity. Further studies are required to characterize this protein. Crude venom from S. verrucosa was also subjected to mitogenic, cytotoxic and flow cytometric analysis, using different cell lines. The venom proteins were fractionated on Sephadex G-200 and RP-HPLC. Two low molecular mass proteins were found to be homogenous on SDS PAGE. Amino acid analysis of these proteins revealed close resemblance to lectins isolated from marine venoms. Further studies are required to characterize the biological activities of these fractions. |
| Download Full Thesis |  1051.93 KB |
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| S. No. | Chapter | Title of the Chapters | Page | Size (KB) |
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| 1 | 0 | Contents | 128.05 KB |
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| 2 | 1 | Introduction | 338.03 KB |
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| 1.1 | Venomous Fish | 2 | ||
| 1.2 | Biochemical Studies On Venoms | 3 | ||
| 1.3 | Cytotoxicity Of Venoms | 4 | ||
| 1.4 | Mitogenic Toxins | 8 | ||
| 1.5 | Pharmacological Activities | 10 | ||
| 1.6 | Enzymatic Activities | 12 | ||
| 1.7 | Hemolytic Activities | 12 | ||
| 1.8 | Scorpionfish | 13 | ||
| 1.9 | Description Of Hypodytes Rubripinnis | 18 | ||
| 1.10 | Description Of Synanceja Verrucosa | 19 | ||
| 1.11 | Objective Of The Current Study | 21 | ||
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| 3 | 2 | Materials And Methods | 85.66 KB |
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| 2.1 | Collection Of Samples | 23 | ||
| 2.2 | Extraction Of Venom | 23 | ||
| 2.3 | Protein Estimation | 23 | ||
| 2.4 | Electrophoresis | 24 | ||
| 2.5 | Gel Filtration | 24 | ||
| 2.6 | High Performance Liquid Chromatography | 25 | ||
| 2.7 | Amino Acid Analysis | 25 | ||
| 2.8 | Agglutinating Activity | 26 | ||
| 2.9 | Mitogenic Activity | 26 | ||
| 2.10 | Cytotoxic Activity | 28 | ||
| 2.11 | Cell Flow Cytometry | 29 | ||
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| 4 | 3 | Results | 352.09 KB |
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| 3.1 | Characterization Of Hypodytes Rubripinnis Venom | 33 | ||
| 3.2 | Characterization Of Synanceja Verrucosa Venom | 56 | ||
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| 5 | 4 | Discussion | 111.03 KB |
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| 4.1 | Hypodytes Rubripinnis | 74 | ||
| 4.2 | Synanceja Verrucosa | 81 | ||
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| 6 | 5 | Conclusions And Future Aspects | 87 | 18.37 KB |
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| 7 | 6 | References | 89 | 174.69 KB |
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