I= EFFECTS OF ANTIBIOTICS ON MOTILITY; SPERM MORPHOLOGY, MEMBRANE INTEGRITY, FERTILITY AND BACTERIOLOGICAL QUALITY OF BUFFALO SPERMATOZOA
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Title of Thesis
EFFECTS OF ANTIBIOTICS ON MOTILITY; SPERM MORPHOLOGY, MEMBRANE INTEGRITY, FERTILITY AND BACTERIOLOGICAL QUALITY OF BUFFALO SPERMATOZOA

Author(s)
Sayed Murtaza Hassan Andrabi
Institute/University/Department Details
Department of Animal Sciences/ Faculty of Biological Sciences/ Quaid-i-Azam University Islamabad
Session
2007
Subject
Biological Sciences
Number of Pages
150
Keywords (Extracted from title, table of contents and abstract of thesis)
motility, sperm morphology, membrane integrity, fertility, buffalo spermatozoa, bubalus bubalis, bull semen, gentamycin, tylosin, linco-spectin, bacterial counts

Abstract
The main objectives of this study were to determine the (1) bacterial species incriminated in buffalo (Bubalus bubalis) bull semen, (2) efficacy of a new antibiotic combination, Le. GTLS (gentamycin, tylosin and linco-spectin) in extender on bacterial counts and appearance of bacterial genera in buffalo semen at different stages of cryopreservation, and in vitro drug sensitivity against the bacteria isolated from fresh semen, (3) effect of GTLS in extender on post-thaw motilities, motion characteristics, plasma membrane integrity, general and acrosomal morphology of buffalo bull spermatozoa, and (4) suitability of GTLS in extender for improvement in fertility of deep-frozen buffalo bull spermatozoa to obtain better pregnancy rate through artificial insemination. In experiment 1, a total of eleven bacterial species were identified from ejaculates of four adult buffalo bulls. The bacterial isolates identified with their frequency of occurrence (%) were; Staphylococcus aureus (16%), Staphylococcus intermedius (11 %), Staphylococcus coagulase (8%), Staphylococcus epidermis (10%), Escherichia coli sp. (16%), Pseudomonas aeruginosa (13%), Pseudomonas putida (2%), Pseudomonas testosterone (4%), Micrococcus sp. (11 %), Proteus mirabilis (6%) and Bacillus sp. (4%). In experiment 2, total aerobic bacterial counts (cfu/ml; median) in post-thaw samples were lower (P<0.05) in GTLS (0.00) than in SP (streptomycin and penicillin; 0.88) or NC (negative control; 2.78). Fewer bacterial genera were identified in semen samples treated with GTLS. They were staphylococcus, micrococcus and E. coli. In addition to these three genera, pseudomonas, proteus and bacillus were observed in SP treated samples. Majority of the bacterial isolates from fresh semen samples showed more sensitivity towards gentamycin, tylosin, lincomycin and spectinomycin as compared with streptomycin and penicillin. Susceptibility to lincomycin and spectinomycin was shown by 100% isolates of pseudomonas. In experiment 3, mean motilities (visual, computer-assisted linear and circular), velocities (straight line, average path and curvilinear) and amplitude of lateral head displacement in post-thaw semen samples did not differ (P>0.05) due to antibiotics. Similarly, sperm plasma membrane integrity did not vary (P>0.05). Morphologically abnormal spermatozoa (%) were lower (P<0.05) in GTLS (17.7 ±2.8) and SP (16.9 ±3.1) than in NC (28.2±3.9). Sperm cells possessing normal acrosome (%) were higher (P<0.05) in GTLS (81. ±3.1) and SF' (79.9 ±3.8) than in NC (72.1 ±3.1). In experiment 4, the fertility rates for SP-based vs. GTLS-containing frozen semen of buffalo bull were 42.8 and 55.2%, respectively. The results for GTLS were significantly higher than SP. The fertility rates also differed significantly in the first and second batch of inseminations performed with SP or GTLS-based cryopreserved semen of buffalo bull. In conclusion, bacterial and seminal quality measured by standard laboratory tests and field fertility trial indicates that GTLS combination of antibiotics is more suitable in semen extender for cryopreservation of buffalo bull spermatozoa.

Download Full Thesis
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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
4308.32 KB
2 1 Isolation Of Bacterial Species In Buffalo Bull Semen 35
745.02 KB
  1.1 Abstract 36
  1.2 Introduction 37
  1.3 Materials And Methods 39
  1.4 Results 42
  1.5 Discussion 42
3 2 Effects Of A New Antibiotic Combination On Bacterial Counts And Appearance Of Bacterial Genera In Buffalo Semen At Different Stages Of Cryopreservation, And In Vitro Drug Sensitivity Against The Bacterial Isolated From Fresh Ejaculates 45
2654.82 KB
4 2.1 Abstract 46
2654.82 KB
  2.2 Introduction 48
  2.3 Materials And Methods 50
  2.4 Results 54
  2.5 Discussion 59
5 3 Effect Of A New Antibiotic Combination On Post-Thaw Motilities, Motion Characteristics, Membrane Integrity, And Morphology Of Buffalo Bull Spermatozoa 62
1697.52 KB
  3.1 Abstract 63
  3.2 Introduction 65
  3.3 Materials And Methods 67
  3.4 Results 71
  3.5 Discussion 78
6 4 Effect Of A New Antibiotic Combination On Fertility Of Frozen Buffalo Bull Semen 84
1124.25 KB
  4.1 Abstract 84
  4.2 Introduction 86
  4.3 Materials And Methods 89
  4.4 Results 93
  4.5 Discussion 96
7 5 General Discussion 99
4668.54 KB
  5.1 References 109