I= ADAPTATION OF A LOCAL WILD INFECTIOUS BURSAL DISEASE VIRUS ON CHICKEN EMBRYO FIBROBLAST CELL CULTURE & EVALUATION OF THE ADAPTED VIRUS AS VACCINE IN CHICKENS
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Title of Thesis
ADAPTATION OF A LOCAL WILD INFECTIOUS BURSAL DISEASE VIRUS ON CHICKEN EMBRYO FIBROBLAST CELL CULTURE & EVALUATION OF THE ADAPTED VIRUS AS VACCINE IN CHICKENS

Author(s)
Muhammad Azhar Khan
Institute/University/Department Details
Faculty of Veterinary Sciences/ University of Agriculture Faisalabad
Session
2005
Subject
Veterinary Microbiology
Number of Pages
110
Keywords (Extracted from title, table of contents and abstract of thesis)
infectious bursal disease virus, chicken embryo fibroblast, chicken, bursae, humoral immune response

Abstract
The present work was carried out to adapt a local wild infectious bursal disease virus (IBDV) on chicken embryo fibroblasts (CEFs) cell culture and to evaluate the adapted virus as vaccine in chicken. The IBD Virus was successfully isolated from field outbreak and identified through agar gel precipitation test (AGPT) and counter current immunoelectrophoresis (CCIE). The positive samples (Bursae) were pooled and purified through chloroform treatment and sucrose gradient centrifugation technique. The infectivity of the purified IBDV was found (EID50) as 104.84/0.2 ml. The isolated IBDV was passaged on CEFs. The virus was adapted on CEFs after 13th Passage and reverse passive haemagglutination (RPHA) virus titre was found as 1:1024. Three experimental vaccines were prepared from the CEFs adapted Local IBOV. The live attenuated, binary elthyleneimine inactivated vaccine (BIV) without adjuvant and BEl inactivated oil adjuvanted vaccine (OAV). All the experimental vaccines were evaluated and compared on the basis of humoral immune response measured through indirect haemagglutination (IHA) test, cellelular immune response measured through splenic cell migration inhibition test, and protection against challenge exposure. Thirty birds from each group were challenged two weeks post vaccination with the virulent field IBDV strain with EID50 of 104.84@ 0.2ml S/C route. Clinical signs and mortality were recorded upto 10 days post inoculation.

The best humoral immune response was recorded in the birds of group G5, whereas the best cellular immune response were exhibited in birds of group G1. The post challenge mortality recorded in groups G1, G2, G3, G4, G5 and G6 was 53.33%, 10%, 6.67%, 43.34%, 3.34% and 86.67% respectively. The maximum protection against challenge exposure was (97 %) as recorded in group G5.

It was evident from the findings of the present study, that local IBDV belongs to very virulent strain and it was adapted and attenuated on CEF after 13th passages. The vaccines prepared from locally attenuated virus were more immunogenic and safer as compared to commercial vaccines. It may be recommended that the experimental vaccines (LAV, BIV and OAV) under control experiment condition may also be tested under field conditions.

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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
225.18 KB
2 1 Introduction 1
92.3 KB
3 2 Review Of Literature 5
539.58 KB
4 3 Materials And Methods 27
373.23 KB
5 4 Results 45
385.49 KB
6 5 Discussion 67
320.04 KB
7 6 Summary 80
621.47 KB
  6.1 Literature Cited 82
  6.2 Appendices 104