I= PHYCOCHEMISTRY AND BIOACTIVITY OF SOME ALGAE FROM MIANI HOR, BALOCHISTAN
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Title of Thesis
PHYCOCHEMISTRY AND BIOACTIVITY OF SOME ALGAE FROM MIANI HOR, BALOCHISTAN

Author(s)
Javed Aftab
Institute/University/Department Details
Centre of Excellence in Marine Biology/ University of Karachi
Session
2006
Subject
Marine Biology
Number of Pages
367
Keywords (Extracted from title, table of contents and abstract of thesis)
algae, miani hor, balochistan, microcystis aeruginosa, lyngbya majuscula, lyngbya martensiana meneghini, oscillatoria princeps vaucher, gloeotrichia raciborskii woloszynska, microspora floccosa, spirogyra hyalina cleve , spirogyra rhizoides rand, cladophora glomera ta, cladophora okamurai, chara zeylanica klein, nitella hyalina

Abstract
Twelve species of estuarine algae were collected from Miani Hor, Balochistan (Pakistan) and their methanol extracts investigated. A variety of 22 saturated and 42 unsaturated fatty acids (F As) were quantitatively analysed as their methyl esters by GLC and GC-MS techniques. Most of the species contained unsaturated F As in greater proportion than the saturated ones. The saturated F As ranged from C5 to C29, among which C16:0 and C17:0 were most prevalent and palmitic acid was the most dominant FA. The unsaturated F As included 15 mono-, 9 di-, 11 tri- and 7 poly-unsaturated ones, among which oleic acid was the most dominant FA. The monounsaturated F As ranged from C8 to C23 and diunsaturated ones from C 11 to C 19. The triunsaturated F As ranged from Cl0 to C29 and polyunsaturated ones (PUFAs) from C12 to C20, among which C17:3 was most common. The PUFAs were detected with 4 - 6 double bonds. The FAs of C 12, C 14, C 16 and C 18 were most common as they were found in all categories of FAs.

Apart from that 18 different terpenes, 14 sterols, 3 hydrocarbon derivatives, 2 carbohydrates and 2 glycosides were isolated, purified and chemically elucidated through IR & UV, El-, FAB-, FD-, GC- & HR-MS and 1H- & 13C-NMR spectroscopy. Terpenes included 1 mono-, 1 sesqui-, 8 di- and 8 tri-terpenes, among which Trans-phytol was most common. The sterols contained 9 ”5-, two ”7-, one ”5, 7- and 2 other types of sterols having cholesta- and stigmasta-skeleton, among which Cholesterol was most common. Hydrocarbons were dihydroxy and isoprenoid derivatives, carbohydrates included di- and poly-saccharides and the glycosides were sterol galactosides.

Crude extracts were tested against 6 species of Gram +ve and 8 of Gram €“ve bacteria, 7 human pathogens, 8 saprophytes and 5 plant pathogens. All the tested organisms were found to be equally susceptible against all the algal species. A variable cytotoxic activity was exhibited by different algal extracts. Members of the three investigated phyla of algae differed from one another in a variety of ways in various aspects of bioactivity.

Download Full Thesis
6869.46 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
577.31 KB
2 1 Introduction 34
188.16 KB
3 2 Materials And Methods 41
454.39 KB
  2.1 Collections Of Algal Material 41
  2.2 Isolation Of Fatty Acids 44
  2.3 Separation Of Other Natural Products 46
  2.4 Instrumentation 48
  2.5 Bioactivity Studies 51
4 3 Results 63
4933.63 KB
  3.1 Microcystis Aeruginosa ( Kutzing 1833) Kutzing 1846 66
  3.2 Lyngbya Majuscula (Dillwyn 1809) Harvey 1833 86
  3.3 Lyngbya Martensiana Meneghini 1837 105
  3.4 Oscillatoria Princeps Vaucher 1803 130
  3.5 Gloeotrichia Raciborskii Woloszynska 152
  3.6 Microspora Floccosa ( Vaucher 1803) Thuret 1850 171
  3.7 Spirogyra Hyalina Cleve 1868 188
  3.8 Spirogyra Rhizoides Rand Ha W A 1938 206
  3.9 Cladophora Glomera Ta (Linnaeus 1753) Kutzing 1843 229
  3.10 Cladophora Okamurai (Ueda 1932) Van Den Hoek 1963 248
  3.11 Chara Zeylanica Klein Ex Willdenow 273
  3.12 Nitella Hyalina (De Candolle 1815) C. A. Agardh 1824 295
5 4 General Discussion 317
635.25 KB
  4.1 Fatty Acids 318
  4.2 Terpenes 328
  4.3 Sterols
  4.4 Other Natural Products 333
  4.5 Antimicrobial Activity 336
  4.6 Cytotoxic Activity 343
6 5 Acknowledgements 345
49.12 KB
7 6 Literature Cited 347
439.49 KB