I= ISOLATION AND STRUCTURAL STUDIES ON PHYTOCHEMICALS ACTIVE IN VARIOUS BIOLOGICAL ASSAYS
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Title of Thesis
ISOLATION AND STRUCTURAL STUDIES ON PHYTOCHEMICALS ACTIVE IN VARIOUS BIOLOGICAL ASSAYS

Author(s)
Afshan Begum
Institute/University/Department Details
H.E.J. Research Institute of Chemistry / University of Karachi
Session
2007
Subject
Chemistry
Number of Pages
170
Keywords (Extracted from title, table of contents and abstract of thesis)
phytochemicals, biological assays, lindelofia stylosa, bergenia ciliate, trigonella foenum graecum, anti-glycatio assay

Abstract
This dissertation is divided into three parts. Part A describes the results of phytochemical and bioassay studies on Lindelofia Stylosa (Kar. & Kir.), while the Part B deals with the isolation and characterization of compounds isolated from Bergenia Ciliate (How.) Sternb., and Part C illustrates the chemical constituents of Trigonellafoenum-Graecum L.

PART A :This part of the thesis describes the isolation and structure elucidation of seven new 1-7 and thirteen known glycosides and phenyl polypropanoides 8-20 from Lindelofia stylosa (Kar. & Kir.). The compounds 1-20 were found to posse's immunomoduladatory activity, while compounds 9-19 exhibited a potent anti-oxidant activity (IC50 values between 0.04 to 0.27 µM). The new constituents were identified as 3-O-feruloyl-6'-O-coumaroyl sucrose (1), 3- O-feruloyl-6'-O-caffeoyl sucrose (2), 3,6-O-diferuloyl-l-kestose (3), 3-O-feruloyl-6'-O-coumaroyl-l-kestose (4), 3,6- O-diferuloyl nystose (5), 3-0-feruloyl-6'- O- coumaroyl nystose (6), and ethyllithospermate (7).

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The structures of new compounds 1-7 were deduced on the basis of different spectroscopic techniques, including 1H- and 13C-NMR spectroscopy (HMQC, HMBC, ID-TOCSY, COSY 45°, etc). The known compounds 8-20 were isolated for the first time from Lindelofia stylosa and identified as 3,6'-O-diferuloylsucrose (8), 3-0-feruloyl fructofuranosyl sucrose (9), dimethyllithospermate (10), dimethyllithospermate B (11), rosmarinic acid (12), methyl rosmarinate (13), ethyl rosmarinate (14), butyl rosmarinate (15), p-coumaric acid (16), ferulic acid (17), 8-O-,B-D-glucopyranosyl torachrysone (18), naphthalene glycoside (8-O-,B-D-glucopyranoside) (19), and 4'-hydroxy-5-methoxy-6,7- methylenedioxyisoflavone (20).

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PART B :Part B of the thesis describes the phytochemical investigation on Bergenia ciliate (How.) Stemb., which has resulted in the isolation of three known compounds, identified as bergenin (21), p-hydroxybenzoyl bergenin (22), and 11- O-galloyl bergenin (23).

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PARTC : Part C of the thesis describes the isolation of secondary metabolites from Trigonella foenum-gracecum L. This has resulted in the isolation of two known constituents, identified as kaempferol-7 -O-α-rhamnoside (24) and kaempferol-3-O- α -L- rhamnopyranoside-7 -O- α -L-rhamnopyranoside (25)

Download Full Thesis
3373.61 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
306.46 KB
2 1 General Introduction 1
130.2 KB
  1.1 Introduction 1
3 2 Phtyochemical Investigation On Secondary Metabolites From Lindelofia Stylosa ( Kar . & Kir ) 8
2140.17 KB
  2.1 Introduction 8
  2.2 Results And Discussion 24
  2.3 Experimental 87
4 3 Biological Activities On Compounds From Lindelofia Stylosa ( Kar . & Kir ) 115
258.97 KB
  3.1 Anti-Oxidant Activity 115
  3.2 Immunomodulatory Activity 120
5 4 Phytochemical Studies On Bergenia Ciliate (How.) Sternb 127
224.62 KB
  4.1 Introduction 127
  4.2 Results And Discussion 129
  4.3 Experimental 134
6 5 Isolation Of Econdary Metabolites From Trigonella Foenum-Gracecum L. 140
218.36 KB
  5.1 Introduction 140
  5.2 Results And Discussion 143
  5.3 Experimental 148
7 6 Biological Activities On Compounds Isolated From Trigonella Foenum Gracecum L. 153
81.87 KB
  6.1 Anti-Glycatio Assay 153
8 7 References 157
93.29 KB
9 8 Glossary 162
134.05 KB
10 9 List Of Publications 170
11.97 KB