I= EPIDEMOLOGICAL, MICROBIOLOGICAL AND MOLECULAR BIOLOGICAL STUDIES OF ENTEROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM DIARRHOEAL PATIENTS OF RAWALPINDI-ISLAMABAD
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Title of Thesis
EPIDEMOLOGICAL, MICROBIOLOGICAL AND MOLECULAR BIOLOGICAL STUDIES OF ENTEROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM DIARRHOEAL PATIENTS OF RAWALPINDI-ISLAMABAD

Author(s)
Abdul Qadar Khan Mohmand
Institute/University/Department Details
Department of Biological Sciences/ Quaid-i-Azam University, Islamabad
Session
1996
Subject
Microbiology
Number of Pages
188
Keywords (Extracted from title, table of contents and abstract of thesis)
enteropathogenic escherichia coli, infantile diarrhoea, gastroenteritis, epec

Abstract
The present study was undertaken in order to understand the basis of prevailing pattern of epidemiology of infantile diarrhoea amongst the population of cities of Rawalpindi-Islamabad. In the first part of the study data on 27159 diarrhoeal gastroenteritis patients of both sexes were recorded in the out-door patient departments in the four hospitals of the twin cities of Rawalpindi-Islamabad, during a period of four years from 1987-90. Out of total patients, 14877 (54.77%) were males and 12282 (45.22%) were females. As far as the distribution is concerned, 17352 patients (63.10%) were recorded from Rawalpindi while the remaining 9807 (36.10%) were from Islamabad. The highest incidence was recorded amongst the age group 0-12 months, with the number of diarrhoeal patients being 14936 (54.99%) followed by the age group 1-2 years where the number of patients remained 8896 (32.75%). The lowest incidence was recorded in the age group of 2-3 years where the number of patients recorded was 3327 (12.25%). As far as intensity of diarrhoea and gastroenteritis is concerned, out of a total of 27159 patients recorded in four years, 8573 (31.56%) were suffering from gastroenteritis and 18586 (68.43%) were suffering from diarrhoea. Breast feeding data revealed that out of the 27159 diarrhoea/ gastroenteritis patients, 11522 (42.42%) were breast-fed and 15637 (57.57%) were fed on other food. The incidence of infection in breast fed patients was low. The second part of the study involved correlating prevalence of enteropathogenic E. coli with infantile diarrhoea and determination of levels of antibiotic resistance amongst these isolates. For this purpose one hundred strains from diarrhoea and gastroenteritis patients falling within 0-3 years of age (after confirmation by biochemical and serological tests) were set aside and screened for levels of susceptibility against 13 conventional antibiotics. All isolates were 100% resistant to ampicillin, bacitracin, penicillin and trimethoprim, while 91 % were resistant to streptomycin, 88% were resistant to tetracycline, 69% to chloramphenicol, 47% to spectinomycin, 14% to rifampicin and 0% to amikacine and gentamicin. Whole cell protein profile analysis of these isolates was carried out to identify the genetic variability by the gel profile. The patterns were compared for variability among the whole cell protein profile by using the method of Dice (1945). Using this method of correlation, it was observed that several groups were distinguishable amongst the isolates. Such data suggest that different immunogenic groups are prevalent in our geogrpahical area.

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1867.92 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents 0
121.73 KB
2 1 Introduction 3
220.25 KB
  1.1 Significance of Diarrhoea 3-4
  1.2 Diarrhoea - Definition and Symptoms 4-6
  1.3 Infantile Diarrhoea 7
  1.4 Outbreaks in Pakistan 8
  1.5 Causative Organism 9-11
  1.6 Mechanism of Diarrhoea 11-12
  1.7 Enteropathogenic E. coli 12-14
  1.8 Enteropathogenic (EPEC) Serotype of E. Coli 14-15
  1.9 Resistance to Antibiotic and Factors of Resistance X. 15-19
  1.10 Molecular Data 19-21
  1.11 Vaccines 21-23
3 2 Materials and Methods 24
361.4 KB
  2.1 Epidemiology of Diarrhoeal Patients 24
  2.2 Microbiology 24
  2.3 Minimum Inhibition Concentration (MIC)/ Antibiotic Resistance 32
4 3 Results 52
1166.3 KB
  3.1 Epidemiological Data-A survey of Diarrhoeal patients 52-57
  3.2 lsolaiton of Escherichia coli. 57-62
  3.3 Minimum Inhibition concentration 62 ,68 -69
  3.4 Whole cell protein profile 127-129
5 4 Discussion 157-162
207.51 KB
  4.1 Reference 164-175