I= HAZARDS OF INSECTICIDES: THE EFFECT OF DICHLORVOS ON DEVELOPING TESTES OF FAYOUMI CHICKS
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Title of Thesis
HAZARDS OF INSECTICIDES: THE EFFECT OF DICHLORVOS ON DEVELOPING TESTES OF FAYOUMI CHICKS

Author(s)
Robina Shaheen
Institute/University/Department Details
Department of Biological Sciences/ Quaid-i-Azam University, Islamabad
Session
2003
Subject
Biology
Number of Pages
101
Keywords (Extracted from title, table of contents and abstract of thesis)
insecticides, developing testes, fayoumi chicks, dichlorvos, ddvp, seminiferous tubule, leydig cell, spermatogenic cells, irregular sertoli cells, spermatocytes, spermatozoa, spermatids

Abstract
Present study was conducted to investigate the effect of dichlorvos (2, 2-dichlorovinyl dimethylphosphate) or DDVP, an organophosphate insecticide at low (4 mg/kg = T1) and high (8 mg/kg = T2) dose, on gross morphology and histomorphology of testes, in young (10 weeks) and adult (13 weeks) Fayoumi chicks. There was no significant (p>O.05) change in body weight of 10 weeks old chicks at low dose (4 mg/kg) but it increased significantly (pO.05) at low as well as high dose of insecticide. Testes weight and size increased significantly (p

Seminiferous tubule diameter and epithelial height in treated groups increased significantly (pO.05) change in Leydig cell nuclear diameter of young and adult chicks was observed.

Histomorphologically studied parameters revealed that the intertubular spaces as well as the interstitial tissue increased in dichlorvos treated testes of 10 and 13 weeks old chicks at low (T1) and high (T2) dose. Leydig cells were present singly in the interstitium.

Round or oval and elongated seminiferous tubules of variable size were scattered throughout the testicular tissue, which were loosely arranged in the center and compactly arranged towards the periphery in low and high dose of dichlorvos treated birds of young and adult age compared to control groups.

Organization of seminiferous epithelium appeared same in testes of all the dichlorvos treated and control groups at 10 weeks of age. It consisted of spermatogenic cells and irregular Sertoli cells. In 10 weeks old testes only spermatogonia of A and B- type were observed and their number was significantly (p

Morphology of Sertoli cells, in treated groups (T1 and T2) remained unaffected compared to control in both young (10 wk) and adult (13 wk) birds. Leydig cells morphology revealed that dichlorvos at low and high dose level caused no significant (p> 0.05) change in chicks of both age groups. Comb size and serum cholesterol levels in 10 and 13 weeks old birds fed with dichlorvos supplemented feed, indicated no sign of toxicity.

No mortality was observed in birds (fed with dichlorvos supplemented feed) for three weeks in 10 and 13 weeks of age. Other morphological characters which includes beak, size of eye ball, phalanges, plumage, combs and wattles were normal in appearance compared to control birds of young and adult age.

It is concluded that intensity of morphological changes (body, testicular and liver weight, testicular and comb size, serum cholesterol levels) as well as histological changes investigated in this study due to the use of this insecticide are dose dependent i.e. low and high, and age of the chicks.

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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents 0
142.03 KB
2 1 Introduction 1
193.13 KB
3 2 Materials And Methods 17
57.59 KB
4 3 Results 24
753.24 KB
5 4 Discussion 63
91.43 KB
6 5 References 72
137.36 KB