 |
| Pakistan Research Repository | Home |
Title of Thesis |
| Author(s) Aftab Ahmed |
| Institute/University/Department Details University of the Punjab |
| Session 1994 |
| Subject Chemistry |
| Number of Pages 168 |
| Keywords (Extracted from title, table of contents and abstract of thesis) rat kidney fibroblasts, nrk fibroblasts, immunioprecipitation, polyacrylamide gel electrophoresis, estimation of dna , tgf-β treatment, actinomycin-d, cytoskeleton |
Abstract |
| Download Full Thesis |  1520.48 KB |
 |
|
|
|
|
| S. No. | Chapter | Title of the Chapters | Page | Size (KB) |
|
|
|
|
|
| 1 | 0 | Content | 62.05 KB |
|
|
|
|
|
|
| 2 | 1 | Introduction And Review Of Literature | 1 | 388.96 KB |
|
|
|
|
|
| 3 | 2 | Materials And Methods | 36 | 156.2 KB |
| 2.1 | Culture Of Normal Rat Kidney Fibroblasts | 36 | ||
| 2.2 | Transfection Of Nrk Fibroblasts With Dna-Sw480 Cells | 36 | ||
| 2.3 | Preparation Of Protein Samples | 39 | ||
| 2.4 | Determination Of Labeled Proteins | 41 | ||
| 2.5 | Polyacrylamide Gel Electrophoresis | 42 | ||
| 2.6 | Immunioprecipitation | 44 | ||
| 2.7 | Assesment Of Actin Filaments | 45 | ||
| 2.8 | Estimation Of Dna Contents | 45 | ||
| 2.9 | Isolation Of Rna | 46 | ||
| 2.10 | Northern Hybridization | 47 | ||
| 2.11 | Evaluation Of Fell Proliferation | 49 | ||
| 2.12 | Differential Typsin Sensitivity Assay | 49 | ||
|
|
|
|
|
| 4 | 3 | Results | 50 | 581.9 KB |
| 3.1 | Expression Of P52 Secreted Protein | 50 | ||
| 3.2 | Effect Of Duration Of Tgf -Î’ Treatment | 53 | ||
| 3.3 | Expression Of P52 Isoforms | 60 | ||
| 3.4 | Expression Of P52 Protein In Total Cell Lysates | 70 | ||
| 3.5 | Expression Of P52 In Substrate Associated Proteins | 76 | ||
| 3.6 | Effect Of Actinomycin -D On P52 | 76 | ||
| 3.7 | Organization Of Actin Filaments | 79 | ||
| 3.8 | Differential Substrate Adhesion Of Fibrtoblasts | 87 | ||
| 3.9 | Confirmation Of P52( Pai-1) | 92 | ||
| 3.10 | Total Dna Contents Of Fibroblasts | 98 | ||
| 3.11 | Pai-1(P52) M Rna Transcription | 101 | ||
|
|
|
|
|
| 5 | 4 | Discussion | 106 | 183.48 KB |
| 4.1 | Tgf-Î’ Regulate The Induction Of Pai-1 | 107 | ||
| 4.2 | Effect Of Tgf - Î’ On Cytoskeleton | 117 | ||
|
|
|
|
|
| 6 | 5 | Bibliography | 123 | 334.22 KB |
|
|
|
|
|