I= BIOCHEMICAL AND MOLECULAR AND ANALYSIS OF THE INDUCTION OF P52 (PAI-1) GENE EXPRESSION BY TRANSFORMING GROWTH FACTOR BETA (TGF-’) IN CULTURED NORMAL RAT KIDNEY FIBROBLASTS
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Title of Thesis
BIOCHEMICAL AND MOLECULAR AND ANALYSIS OF THE INDUCTION OF P52 (PAI-1) GENE EXPRESSION BY TRANSFORMING GROWTH FACTOR BETA (TGF-’) IN CULTURED NORMAL RAT KIDNEY FIBROBLASTS

Author(s)
Aftab Ahmed
Institute/University/Department Details
University of the Punjab
Session
1994
Subject
Chemistry
Number of Pages
168
Keywords (Extracted from title, table of contents and abstract of thesis)
rat kidney fibroblasts, nrk fibroblasts, immunioprecipitation, polyacrylamide gel electrophoresis, estimation of dna , tgf-β treatment, actinomycin-d, cytoskeleton

Abstract
No Abstract

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1520.48 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Content
62.05 KB
2 1 Introduction And Review Of Literature 1
388.96 KB
3 2 Materials And Methods 36
156.2 KB
  2.1 Culture Of Normal Rat Kidney Fibroblasts 36
  2.2 Transfection Of Nrk Fibroblasts With Dna-Sw480 Cells 36
  2.3 Preparation Of Protein Samples 39
  2.4 Determination Of Labeled Proteins 41
  2.5 Polyacrylamide Gel Electrophoresis 42
  2.6 Immunioprecipitation 44
  2.7 Assesment Of Actin Filaments 45
  2.8 Estimation Of Dna Contents 45
  2.9 Isolation Of Rna 46
  2.10 Northern Hybridization 47
  2.11 Evaluation Of Fell Proliferation 49
  2.12 Differential Typsin Sensitivity Assay 49
4 3 Results 50
581.9 KB
  3.1 Expression Of P52 Secreted Protein 50
  3.2 Effect Of Duration Of Tgf -’ Treatment 53
  3.3 Expression Of P52 Isoforms 60
  3.4 Expression Of P52 Protein In Total Cell Lysates 70
  3.5 Expression Of P52 In Substrate Associated Proteins 76
  3.6 Effect Of Actinomycin -D On P52 76
  3.7 Organization Of Actin Filaments 79
  3.8 Differential Substrate Adhesion Of Fibrtoblasts 87
  3.9 Confirmation Of P52( Pai-1) 92
  3.10 Total Dna Contents Of Fibroblasts 98
  3.11 Pai-1(P52) M Rna Transcription 101
5 4 Discussion 106
183.48 KB
  4.1 Tgf-’ Regulate The Induction Of Pai-1 107
  4.2 Effect Of Tgf - ’ On Cytoskeleton 117
6 5 Bibliography 123
334.22 KB