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Title of Thesis

Rahila Najam
Institute/University/Department Details
University of Karachi, Pakistan
Number of Pages
Keywords (Extracted from title, table of contents and abstract of thesis)
pharmacolgical screening, bioactive products, marine resources, human brain, home cage activity, open field activity, estimation of indolamines, catecholamines, hematological parameters, seaweeds, algae flora,

The exploration of seaweeds for commercial utilization is under extensive investigations since the world is facing the damage of the shortage of conventional food. Similarly the utilization of seaweeds as a potential source of medicine could also contribute significantly in this field. The exploration of seaweeds as a source of medicine is under progress and there are many reports in which different pharmacological properties have been investigated and reported. Pakistan has rich algae flora in the coastal and in shore waters of northern Arabian sea (Shameel and Tanaka, 1992), and several studies have been made on the phytochemistry of seaweeds during last decade (Bano et aI., 1988, Ahmed and Shameel, 1990).

Among important divisions of seaweeds the members of chlorphyte are less extensively investigated and found to have various important products (Amjad and Shameel, 1993). The isolation and 13C NMR spectroscopy of the water soluble polysaccharides from south Arabia Red algae was done. (Furwax and Miller, 1986). Many other types of seaweed are considered as vary good source of some bioactive products and according to Maryama et al., 1972 there may be possible preventive effect of dietary seaweeds as internal radiation injury of the thyroid by radioactive iodine.

Keeping in mind the importance of seaweeds and other marine resources, present study was designed to evaluate the pharmacological activities of some marine sources collected from Karachi coast.

The collection of seaweeds was carried out from Paradise Point and Manora of Karachi. The identification phase was followed after collection. The collected seaweeds were drained of seawater and epiphytes as well as the calcarious deposit were removed. Fresh seaweeds were dried completely and then were soaked in methanol (about 2 lit x 3) for thirty days. After thirty days, the extract was filtered through the filter paper and the residue left was the methanolic extract tested for various pharmacological activities.

The seaweeds, which were collected and identified for the study, are 1. Codium iyengarii (green) 2. Sargassem bindrii (brown] 3. Codium ftabellatum (green) 4. Hypnea musciformis (red) The present study is designed to investigate the effects on hematological and, biochemical and on brain biogenic amines. For this purpose the study is carried out on rabbits (for biochemical and hematological) and on albino mice (for brain biogenic amines).

Download Full Thesis
6146.85 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 1 General Introduction
1695.24 KB
  1.1 Classification 3
  1.2 Reproduction And Life Cycle 4
  1.3 Pharmacological Properties 5
  1.4 Human Brain 23
  1.5 Blood 31
2 2 Materials And Methods 37
1056.47 KB
  2.1 Fieldwork 38
  2.2 Dosing Protocol 41
  2.3 Drugs And Injections 44
  2.4 Chemicals 45
  2.5 Acquisition Phase 46
  2.6 Home Cage Activity 46
  2.7 Open Field Activity 47
  2.8 Tissue Sampling Technique 48
  2.9 Brain Dissection Technique 49
  2.10 Neurochemical Estimation 50
  2.11 Materials 52
  2.12 Estimation Of Indolamines And Catecholamines 54
  2.13 Results 60
  2.14 Biochemical Parameters 60
  2.15 Experimental Protocol 60
  2.16 Hematological Parameters 61
  2.17 Experimental Protocol 62
  2.18 Effects Of Seaweeds On Brain Biogenic Amines And Their Metabolites 63
  2.19 Experimental Protocol 63
  2.20 Pharmacological Screening For Cns Activities 64
3 3 Discussion 65
2369.39 KB
  3.1 Future Investigations 79
4 4 References 80
793.4 KB