I= THE REPRODUCTIVE PHYSIOLOGY OF THE TELEOST, COLISA FASCIATA (BLOCH AND SCHNEIDER)
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Title of Thesis
THE REPRODUCTIVE PHYSIOLOGY OF THE TELEOST, COLISA FASCIATA (BLOCH AND SCHNEIDER)

Author(s)
Muhammad Nazir Bhatti
Institute/University/Department Details
University Of The Punjab
Session
1972
Subject
Zoology
Number of Pages
480
Keywords (Extracted from title, table of contents and abstract of thesis)
teleost, colisa fasciata, spermatogenetic cells, thiourea treatment, methallibure, piscine pituitary extracts, mammalian pituitary gonadothropins, vitellogenesis

Abstract
The annual cyclical changes in the gametogenetic activity of gonads of Colisa fasciata (Bloch and Schneider) were studied on the basis of gone somatic index and histology of the gonads. The process of vitellogenesis in the ovary has been associated with the nucleolus like structures, the vitellonucleoli in the nucleus of the oocyte. The secretion of male sex hormones has been attributed to the interstitial cells in the testis. In the ovary, two types of endocrine sources have been observed, i.e., the pre-ovulatory, and 'post-ovulatory ' cor**ra formed by the granulose cells. The cyclical changes in the pituitary gland were studied and the gonad tropic activity was found to be localized in the cyanophilic gonadotrophs in the meso-adenohyophysis. The gonadotrophs were differentiated from the thyrotrophs on the basis of size, the gonadotrophs being larger. The identity of these cells was further confirmed by thiourea treatment which resulted in hypertrophy and hyperplasia throtrophs. The gonadotrophs were observed to show numerical variations in correlation with the gonadal cycles. The pituitary gonadotrophs and the gonads were observed to correspond to the environmental temperature and daylight. The effects of administration of an inhibitor of pituitary gonadtroins, methallibure, I.C.I. 33.828 (a dithiocarbamoylhydrazine derivative) on the gonads and the methallibure was on effective inhibitor of pituitary gonadotropins in colisa fasciata also.

A comparison was made of the effects of piscine pituitary extracts, and purified mammalian pituitary gonadotropins on the methallibure treated as well as normal fish. The pituitary extracts were found to be more effective than the purified mammalian pituitary hormones. The methallibure treated animals gave more conclusive results than the normal ones. FSH alone produced no effects on the pituitary and gonads, whereas LH produced significant activation of the gonads. The administration of FSH and LH combined (1:1) produced more significant stimulation of pituitary and gonada than LH alien. The identity of the pituitary gonadotropins of the fish understudy has been discussed.

Download Full Thesis
12565.78 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
748.58 KB
2 1 General Introduction 1
117.28 KB
3 2 Material And Methods 6
595.65 KB
  2.1 Animals 6
  2.2 Procedure For Gonads 9
  2.3 Gonosomatic Index 20
4 3 Cyclical Changes In The Testicular Activity 28
1230.39 KB
  3.1 Introduction 29
  3.2 Results 32
  3.3 General Morphology 32
  3.4 Histological Structure 33
  3.5 Degeneration Of Various Spermatogenetic Cells 41
  3.6 Seasonal Changes 41
  3.7 Spawning Behavior 67
  3.8 Discussion 68
5 4 Cyclical Changes In The Ovarian Activity 81
2719.5 KB
  4.1 Introduction 82
  4.2 Results 86
  4.3 General Morphology 86
  4.4 Histological Structure 57
  4.5 Atresia 105
  4.6 Spawning Behaviour 109
  4.7 Spent Follicles 109
  4.8 Seasonal Changes 111
  4.9 Discussion 139
6 5 Cyclical Changes In The Pituitary Gland 168
1004.92 KB
  5.1 Introduction 169
  5.2 Results 171
  5.3 General Morphology 171
  5.4 Histological Structure 171
  5.5 Cyclical Changes 179
  5.6 Discussion 196
7 6 Effects Of Thiourea Treatment On The Hypophysis And Gonads 208
719.49 KB
  6.1 Introduction 209
  6.2 Methods 211
  6.3 Results 212
  6.4 Discussion 219
  6.5 Correlation Of Gonadal Cycles With The Pituitary Cycle 223
  6.6 Correlation Of Temperature And Day Length With The Reproductive Cycles 226
8 7 The Effects Of Methallibure , Piscine Pituitary Extracts And Mammalian Pituitary Gonadothropins Administration Of The Hypophysis And Gonads Of The Normal And Methallibure Treated Fish
1714.7 KB
  7.1 Introduction 238
  7.2 Methods 243
  7.3 Results 251
  7.4 Discussion 282
9 8 Effects Of Mammalian Gonadal Steroids Administration On The Fish:
302.4 KB
  8.1 Introduction 297
  8.2 Methods 298
  8.3 Results 299
  8.4 Discussion 207
10 9 Age Changes In The Pituitary Gland And Gonads : 312
265.24 KB
  9.1 Introduction 303
  9.2 Methods 314
  9.3 Results 316
  9.4 Discussion 326
11 10 General Discussion 325
3068.11 KB
  10.1 Summary 335
  10.2 References 343
  10.3 Appendices 388