I= ADAPTIVE REPAIR IN MICROCOCCUS LUTEUS
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Title of Thesis
ADAPTIVE REPAIR IN MICROCOCCUS LUTEUS

Author(s)
Muhammad Amin Athar
Institute/University/Department Details
University of the Punjab, Lahore
Session
1991
Subject
Molecular Biology
Number of Pages
155
Keywords (Extracted from title, table of contents and abstract of thesis)
micrococcus luteus, dna, excision repair, mismatch repair, adaptive repair, n-nitroso, dna repair, oxidative stress, n-nitroso compounds, carcinogenesis, dna alkylation

Abstract
No abstract

Download Full Thesis
1305.72 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
146.58 KB
2 1 Introduction 1
39.17 KB
3 2 Historical Perspective Of DNA Repair 4
407.71 KB
  2.1 Photoreactivation 4
  2.2 Excision Repair 5
  2.3 Post Replication Repair 9
  2.4 Repair Of Crosslinks 9
  2.5 SOS Response 9
  2.6 Mismatch Repair 11
  2.7 Adaptive Response To Oxidative Stress 14
  2.8 Adaptive Repair 14
  2.9 Role Of N- Nitroso Compounds In Carcinogenesis 31
  2.10 Effects Of DNA Alkylation 35
4 3 Materials And Methods 43
99.31 KB
  3.1 Bacterial Strains And Media 43
  3.2 Radiochemical And Mutagens 43
  3.3 Chemicals And Reagents 43
  3.4 Preparation Of Pyrimidine Authentic Markers 44
  3.5 Alkylation Of DNA 44
  3.6 Methylation Of Homopolymers 45
  3.7 Adaptation 45
  3.8 Cell Survival 46
  3.9 Measurement Of The Removal Of Modified Bases From Cellular DNA 46
  3.10 Preparation Of Crude Protein Extract 46
  3.11 In Vitro Removal Of Modified Basis From Alkylated DNA 47
  3.12 Transfer Of Methyl Groups 47
  3.13 Measurement Of The Removal Of Modified Pyrimidines 47
  3.14 Amino Acid Analysis Of Methylated Proteins 49
  3.15 Purification Of DNA Glycosylase Enzymes 49
5 4 Results 51
440.57 KB
  4.1 Induction Of Increased Resistance To The Killing Effects Of Alklating Chemical 51
  4.2 Cellular Repair Of Alkylated Basis 54
  4.3 In Vitro Repair Of Alkylated Basis 54
  4.4 In Vitro Repair Of O- Methylated Pyrimidines 57
  4.5 Repair Of O 4 €“ Methyllthymine By DNA Methylatransferase 59
  4.6 Identification Of O- Methylpyrimindine Repair Enzymes 65
  4.7 Purification Of Glycosylase Enzymes 69
  4.8 Purification Of Methyltransferases 81
6 5 Discussion 96
99.13 KB
7 6 References 105
244.31 KB