I= BODY COMPOSITION, ELEMENTAL CONCENTRATION AND MORPHOMETRICS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) AND BIG-HEAD CARP (ARISTICHTHYS NOBILIS) IN PAKISTAN.
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Title of Thesis
BODY COMPOSITION, ELEMENTAL CONCENTRATION AND MORPHOMETRICS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) AND BIG-HEAD CARP (ARISTICHTHYS NOBILIS) IN PAKISTAN.

Author(s)
Muhammad Naeem.
Institute/University/Department Details
Bahauddin Zakariya University, Multan/ Institute of Pure and Applied Biology
Session
2002
Subject
Biology
Number of Pages
133.
Keywords (Extracted from title, table of contents and abstract of thesis)
rainbow trout, oncorhynchus mykiss, big-head carp, aristichthys nobilis, head length, body depth, pectoral fin length, dorsal fin length, tail length, tail width, total length, body weight

Abstract
Eighty four farmed Rainbow trout, Oncorhynchus mykiss and Eighty one Bighead carp, Aristichthys nobilis of different body sizes were selected for analysis of body composition parameters and morphometric variables in relation to body size and condition factor out of which eighty farmed Rainbow trout and Seventy one farmed Bighead carp were used for the analysis of trace metal concentrations in relation to body size.

Each fish was measured, weighed, dried and powdered for the analysis of water content, ash content, fat content and protein content. It was observed that highly significant inverse correlations exist between water content (%) and fat content (% wet weight) (r =-0.937); protein content (% wet weight) (r =0.704), protein content (% wet weight) (r=0.746) and negative influence on water content (%) (r=-0.734). Condition factor has a significant inverse correlation between water content (%) (r = -0.459), significant positive correlation with fat content (% wet weight) (r = 0.528) and protein content (% wet weight) (r = 0.356). Total length has a positive influence on fat content (% wet weight) (r = 0.702), protein content (% wet weight) (r =0.776) and negative influence on water content (%), (r = -0.775) for Oncorhynchus mykiss. While for Aristichthys nobilis, significant inverse correlation exist between water content (%) and fat content (% wet weight) (r = -0.906); protein content (% wet weight) (R = -0.847). Body weight has a positive influence on fat content (% wet weight) (r = 0.453), protein content (% wet weight) (r = 0.497) and negative influence on fat content (% wet weight) (r = -0.595). Total length has a positive influence on fat content (% wet weight) (r = 0.507), protein content (% wet weight) (r = 0.539) and negative influence on water content (%) (r = -0.649), Condition factor has a significant inverse correlation between water content (%) (r = -0.701), positive correlation with fat content (% wet weight) (r = 0.655); protein content (% wet weight) (r = 0.581). Equations were developed to describe these relationships. If only the water content is determined, the equations provide satisfactory estimates of fat content and protein content. If it is impossible to determine the water content, then body constituents can be determined from the body weight length and condition factor using multiple regression models. As variations in body composition are related to these variables, the equations of each constituent were estimate and were found to be highly significant (P <0.001). the predictive equations can be used to estimate values of body composition with a fair amount of accuracy for both species.

In morphometric studies, head length, body depth, Pectoral fin length, dorsal fin length, tail length, and tail width in relation to total length and body weight of the fish to investigate allometric growth. It was observed that in Oncorhynchus mykiss tail width showed isometric growth, body depth positive allometry while head length, Pectoral fin length, dorsal fin length and tail length negative allometry with increasing total length and body weight. In Aristichthys nobilis head length, dorsal fin length, showed isometric growth, body depth, Pectoral fin length, positive allometry tail length and tail width negative allometry with increasing total length. While head length, pectoral fin length showed isometric growth, body depth positive allometry and dorsal fin length, tail length and tail width negative allometry with increasing body weight. All these relations showed high correlations. Slopes of the log transformed data were used to compare with an isometric slope (b = 1 or b = 0.33).

Flame Atomic Absorption Spectrometry was used as an analytical tool for analysis of trace metal concentration in relation to body size. It was observed that total quantity of these metals i.e. Mn, Cu, Co and Cd were found to increase isometrically while Na, K, Ca, Mg, Fe, Zn, and Pb showed negative allometry with increasing body weight. Mg, Mn, Fe, Cu, Cr, Co, and Cd showed isometric growth with increasing body length for Oncorhynchus mykiss. However Na, K, Ca, Zn and Pb showed negative allometry with increasing body length. In Aristichthys nobilis, Na, K, Ca, Mg, Cu, Zn, Cr, Cd and Pb were found to increase isometrically while Mn, Fe and Co showed positive allometry with increasing body length. However Na, Mn, Fe, Cu, and Co showed positive allometry with increasing body length. Predictive models have been found to be highly significant.

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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents 0
571.35 KB
2 1 Abstrct 1
772.97 KB
  1.1 General introduction 3
  1.2 Aims and objects 11
3 2 Body composition 12
709.58 KB
  2.1 Introduction 12
  2.2 Materials and methods 15
  2.3 Results 17
  2.4 Discussion 41
  2.5 Elemental concentration
 
  2.6 Introduction 55
  2.7 Materials and methods 69
  2.8 Results 75
  2.9 Discussion 85
4 3 Morphometry 89
508.73 KB
  3.1 Introduction 89
  3.2 Materials and methods 95
  3.3 Results 97
  3.4 Discussion 110
5 4 References 115
319.31 KB