Mustard seed (Brassica Juncea) RL-18 variety were found to contain 27.24 % protein, 35.22% oil, 0.85% allylisothiocyanate(AITA) 1.84% phytic acid whereas mustard seed meal i.e. solvent extracted oil seed cake, contained 43% protein, 2.05% oil, 1.22% AIT and 2.75% phytic acid. Detoxification of mustard seed meal resulted in reduction of AI Tot below detectable level but phytic acid content in the meal increased to 3.21% However, detoxification treatment followed by steeping in 4% NaC1 at pH 5 reduced the phytic acid content from 3.21 to 0.45 % Detoxified mustard seed meal and low phytate-detoxified mustard seed meal showed nitrogen solubility profile of 30.12%% nad 39.66% (in water, 10% NaC1 and 0.2 % NaOH whereas in vivo digestibility of dry matter was found to be 75.51 and 86.05 % respectively.
Feeding trials on albino rats showed that not protein utilization values of the isonitrogenous diets containing mustard seed meal (untreated); detoxified mustard seed meal; low phytate-detoxified mustard meal; mustard protein concentrate and casein were 45.52% 64.85 %, 67.11%, 68.50% and 73.20 % whereas protein efficiency ratio were 0.84, 2.15, 2.26, 2.38 and 2.53 respectively.
Four experiments of 8 weeks each were conducted on day-old PIA-Shavar chicks using detoxified mustard seed meal (DMSM) or low phytate-DMSM as a substitute of different level of vegetable as well as animal protein sources i.e. sesame, blood and fish meals. These experiments showed that 100% sesame meal; 66% blood meal; 33% blood and 33% fish meals; 33% blood, 33% fish and 100% sesame meals could be replaced by detoxified mustard seed meal(DMSM) without affecting growth or enlargement of thyroid glands. Incorporation of DMSM in broiler ration improved weight gain, feed intake, feed efficiency, feed cost per kilogram of weight gain and dressing percentage. Substitution of upto 33% blood , 33% fish and 100% sesame meals by low phytate-DMSM showed further improvement in weight gain and efficiency, feed cost per kilogram of weight gain and dressing percentage. However, replacement of higher levels of animal protein i.e 33% fish and 66% blood meals by low phytate-DMSM adversely affected the growth of chicks and feed efficiency.
17 weeks experiment on layer was conducted using detoxified mustard seed meal (DMSM) as a substitute of different levels of sesame, blood, fish and decorticated cotton seed meal. The result showed that 100% sesame meal ; and 100 % decorticated cotton seed meal could be replaced by DMSM in layer rations. Egg production and quality of eggs of the birds kept on the ration containing DMSM was comparable with the control ration. Laying capacity decreased when 33% fish and 100% sesame meals were replaced by DMSM.
Mustard protein concentrate prepared during the present investigations, contained 53.50% protein, 2.68 % oil, 0.43% phytic acid and AIT below detectable level. Fortification of Pakistani traditional dishes with 5% (w/w basis) mustard protein concentrate increased their protein contents from 6.41 to 97.73 % maximum being in rice maroonda‚€™ and minimum in ‚€˜shami kabab‚€™. Organoleptic evaluation tests showed that all the dished after fortification with mustard protein concentrate were readily acceptable.