I= EFFECT OF ORGANIC AND INORGANIC NUTRIENTS AND HORMONES ON GROWTH AND DEVELOPMENT OF TISSUE EXPLANT FROM LOCAL PISTACHIO CULTIVARS CULTURED IN VITRO
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Title of Thesis
EFFECT OF ORGANIC AND INORGANIC NUTRIENTS AND HORMONES ON GROWTH AND DEVELOPMENT OF TISSUE EXPLANT FROM LOCAL PISTACHIO CULTIVARS CULTURED IN VITRO

Author(s)
Shaista Jabeen
Institute/University/Department Details
Institute of Chemistry/ University of the Punjab
Session
1993
Subject
Chemistry
Number of Pages
250
Keywords (Extracted from title, table of contents and abstract of thesis)
tissue explant, pistachio, pistacia vera linn, plant regeneration, cotyledons

Abstract
Procedures have been developed for the plant regeneration through callus culture. Seeds of Pistacia vera Linn. (pistachio) producing aseptic seedlings were effectively sterilized by dipping in 0.1% ( w Iv ) mercuric chloride solution containing few drops of Tween-20 for 15 minutes. Explants were obtained from aseptically grown seedlings. The cotyledons with intact seed-coats had a beneficial effect on proper sterilization as well as germination rate. Chilling of the seeds at 4 °C for 48 h given before sterilization was found helpful in breaking down. dormancy which in turn improved the rate of germination. MS medium in the semi-solid form was found to be superior to all other salt mixtures in terms of seed survival and germination rate. Cotyledonary explants in the presence of 2.0 mg/L each of 2,4-D and KIN and 3% sucrose produced maximum amount of callus with desired characteristics. NH4CI and L-glutamine could not improve callus growth. Reduction of macro-nutrients of full strength MS medium to ½ or ¼ strength showed no beneficial effect on growth of cotyledonary calli. Reduction in vitamin contents of MS medium to ½ improved calli characteristics (colour and texture) and growth. But reduction of Fe-EDTA to half or zero strength had deterious effect on P. vera L. callus. Addition of 2.0 mg/L of 2,4-D and KIN each in the medium was the most suitable combination of growth regulators for callogenesis of cotyledonary explants. Addition of 10% (v/v) coconut milk delayed browning of the calli and improved growth of healthy calli. Morphogenesis was induced in calli grown on medium containing NAA (0.25 mg/L) and BA (2.0 mg/L).Development of regenerated shoot buds into plantlets was achieved on medium containing NAA (0.25 mg/L), BA (2.0 mg/L) and L-glutamine (250 mg/L). from cotyledonary calli Shoot proliferation of the regenerated buds was achieved on medium containing 2.0 mg/L of KIN and 4.0 mg/L of BA.-GA3 could not improve the growth of regenerated shoots. Rhizogenesis of regenerated shoots was not achieved.

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3640.69 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
155.07 KB
2 1 Introduction 1
112.32 KB
3 2 Review Of Literature 15
145.93 KB
4 3 Materials And Methods 30
90.56 KB
  3.1 Plant Material 31
  3.2 Preparation Of Media 31
  3.3 Plant Growth Regulators 32
  3.4 Determination Of Ph Of The Medium 37
  3.5 Sterilization Of The Culture Materials 37
  3.6 Disinfection Of Plant Materials 38
  3.7 Growth Conditions 38
  3.8 Evaluation Of Results 38
5 4 Results 40
2605.72 KB
  4.1 Sterilization And Maintenance Of Plant Materials 41
  4.2 Callus Initiation And Maintenance 61
  4.3 Regeneration Studies 122
  4.4 Rhizogenesis Of P. Vera L. Adventive Shoots 160
6 5 Discussion 170
197.3 KB
7 6 Summary 189
298.08 KB
  6.1 References 193
  6.2 Appendices