I= SOLAR INDUCED SKIN DAMAGE AND PROTECTIVE EFFECT OF ANTI-OXIDANTS IN HAIRLESS MICE AND HUMAN VOLUNTEERS
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Title of Thesis
SOLAR INDUCED SKIN DAMAGE AND PROTECTIVE EFFECT OF ANTI-OXIDANTS IN HAIRLESS MICE AND HUMAN VOLUNTEERS
Author(s)
Neelam Muizzuddin
Institute/University/Department Details
Department of Zoology/ University of the Punjab
Session
1996
Subject
Zoology
Number of Pages
97
Keywords (Extracted from title, table of contents and abstract of thesis)
skin damage, antioxidants, hairless mice, human, erythema, photoaging, carcinogenic effects, free radicals, sunburn

Abstract
The Ultraviolet(UV) component of the solar spectrum is responsible for skin damage manifested in the form of erythema, photoaging as well as carcinogenic effects (Kligman, 1969). Ultraviolet B (280-320 nm) Portion of the solar irradiation causes free radical generation skin. Free radicals are species characterized by an odd number of orbital electrons or by pairs of electrons of similar directional spin isolated singly in separate orbitals. Consequently, most of these agents are highly reactive and usually exhibit an extremely short half life. Due to their highly reactive nature, these species have a potentially damaging effect on skin (Al Barwani and Potten, 1985) resulting in sunburn reactions.

This study was designed to observe, histologically, the damaging effects of Ultraviolet B radiation on various parts of skin like the stratum corneum and the epidermis, and to study the protective effect of anti-oxidants, using an animal model. The possible applications of findings from the animal model was then studied on protection of human skin against the different parts of the solar spectrum; the UV-A and R.

The data from these studies suggest that low level chronic exposures to UV can lead to alteration of the skin like epidermal thickening and appearance of sunburn cells. The data also indicates that a mix of common anti-oxidants and free radical scavengers are photoprotective against chronic skin damage in hairless mousse skin model

Treatment with anti-oxidants prior to exposure to solar irradiation allows protection of human skin against UV-B, UV-A as well as IR. From this study it is clear that anti-oxidant and free radical scavengers can be valuable tools, especially in addition to traditional sunscreens, for protection of skin against the formidable effects of long term chronic actinic exposure, namely skin cancer and photoaging.
Download Full Thesis
1004.96 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
40.06 KB
2 1 Introduction 4
21.03 KB
3 2 Literature review 7
118.47 KB
  2.1 Free radicals and skin 10
  2.2 Anti-oxidants 13
  2.3 Mechanism of action of anti-oxidation 14
  2.4 Sunburn cells 15
  2.5 UV-B 17
  2.6 UV-A 19
  2.7 IR 20
  2.8 Skin Types 22
4 3 Materials and methods 25
674.87 KB
  3.1 Part I: Long term effect of free radical scavengers and anti-oxidants on protection of hairless mouse skin exposed to chronic doses of UV-B 25
  3.2 Part II: Human studies 30
  3.3 Results 36
  3.4 Part I: Long terms effect of radical scavengers and anti-oxidants on protection of hairless mousse skin exposed to chronic doses of UV-B 36
  3.5 Part II Human studies 58
  3.6 Summary : Human Studies 67
5 4 Discussion 68
48.83 KB
  4.1 Sunburn cells 70
  4.2 Epidermal thickness 71
  4.3 The sunburn reaction 73
  4.4 UV-A 74
  4.5 IR 74
6 5 Conclusions 76
93.99 KB
  5.1 References 77