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Title of Thesis  
BIOTECHNOLOGICAL POTENTIAL OF IMMOBILIZED ENZYMES  
Author(s)  
MOHAMMAD YAQOOB  
Institute/University/Department Details  
University of Quetta/Political Science  
Status (Published/ Not Published/ In Press etc)  
Published  
Date of Publishing  
1997  
Subject  
Chemistry  
Number of Pages  
103  
   
Keywords (Extracted from title, table of contents and abstract of thesis)  
Immobilized, Enzymes, Biosurfactants, Lipase, Phospholipids, Phospholipases, Glycerol-3-phosphate (GP), Glycerophosphorylcholine (GPC), Choline, Phosphorylcholine, Mechanical strength, Microbial resistance, Thermal stability, Chemical functionality, Hydrophobic/hydrophilic character, Regeneration, Loading capacity,  

 

 
Abstract  

The analytical and biotechnological applications of enzymes are well known for the selective transformation of substrate to products. Immobilization of enzymes offers the advantage of these catalysts to be separated physically from the mixture of substrate and product for reuse, mechanical strength microbial resistance, thermal stability, chemical functionality, hydrophobic/hydrophilic character ease of regeneration, loading capacity and cost. These solid state catalysts may prove novel for the production of many compounds of interest. The work presented in this thesis is divided into five sections. The first section is devoted to an upto date knowledge on the biotechnological potential of immobilized enzymes, in analysis and synthesis, backed up by a very recent and vast survey of literature. The second section describes experimental work. Followed by an introduction, the isolation and purification to electrophoretic homogeneity of bovine pancreatic lipase, the utilization of this enzyme in an immobilized form for the production of biosurfactants and a flow injection procedure for lipase activity measurement using emulsified substrates is given. The third section deals with the application of phospholipases, in particular phospholipase-D in an immobilized for the production of phosphatidate. The remaining sections i.e., fourth and fifth, phospholipase-D in soluble form coupled with other immobilized enzymes is used for the analysis of substrate of clinical importance, including glycerol-3-phosphate, glycerophosphorylcholine, phosphorylcholine and choline etc. using spectrophotometric and chemiluminescent detection systems. In the end a brief discussion on general conclusions and suggestions for future work are given.

 
   
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Sr.No Chapter Table of Contents
 
i 180.kbs
80.KB
1 1

INTRODUCTION

1
242.KB
2 2 Isolation and purification, immobilization of bovine pancreatic lipase for the production of biosurfactants and measurement of lipase activity on FlA. 24
283.KB
3 3 Production of phosphatidate by immobilized phospholipase-D 53
101.KB
4 4 Chemiluminescent and spectrophotometric determination of glycerol-3-phosphate and glycerophosphorylcholine using soluble and immobilized enzymes 63
142.KB
5 5 Chemiluminescent and spectrophotometric assays for choline, phospholipase-D and phosphorylcholine using immobilized 80
134.KB
6 6 References 97
25.KB
7 7 Conclusions 99
19.KB
8 8 Future Trends 101
22.KB
9 9 Publications 103
14.KB