I= STUDIES ON DETOXIFICATION OF BRASSICA SEEDS TO GET BLAND PROTEIN CONCENTRATE TO FILL THE PROTEIN GAP IN PAKISTAN
Pakistan Research Repository Home
 

Title of Thesis
STUDIES ON DETOXIFICATION OF BRASSICA SEEDS TO GET BLAND PROTEIN CONCENTRATE TO FILL THE PROTEIN GAP IN PAKISTAN

Author(s)
Shaukat Ali
Institute/University/Department Details
Department of Chemistry Foreman Christian College, Lahore
Session
1981
Subject
Chemistry
Number of Pages
215
Keywords (Extracted from title, table of contents and abstract of thesis)
brassica seeds, detoxification, bland protein, brassica juncea, brassica campestris, brassica napus, trace salts, mustard seed, mold, aspergillus niger, aspergillus flavus, mucor mucedo

Abstract
Three varieties of Brassica seeds(Brassica juncea, Brassica campestris, Brassica napus)were studied for their proximate composition and were detoxified by chemical and bio-chemical methods. Composition of Brassica seeds depended upon date of sowing, environmental conditions, such as temperature, drought, length of day, varying fertility levels and composition of the soil. Hull to kernel ratio of Brassica seeds depended on environmental conditions and availability of nutrients to the plant. Protein, oil and allylisothiocyanate contents of Brassica seeds decreased in order of kernels to seeds and then to hulls respectively. Fibre contents of Brassica seeds decreased in order of hulls to seeds and then to kernels respectively. Microbiological evaluation of the amount of the Brassica seed meal showed that a fair total amino acids in the meal was not available

Microorganisms capable of growing on mustard seed cake were isolated from air in the surroundings of P.C.S.I.R. laboratories and identified. Three strains of mold(Aspergillus niger, Aspergillus flavus and Mucor mucedo) and two of bacteria (Bacillus subtilis and Bacillus subtilis var niger) grew well when mustard seed cake was the only source of carbon. Aspergillus flavus also hydrolysed a pure sample of sinigrin(a toxic substance present in the cake) and did not show florescent aflatoxin band. All the locally isolated strains were employed for detoxification of RL-18 cake.

Biological detoxification of RL-18 cake with Aspergillus flavus, Aspergillus niger and Bacillus subtili var niger resulted in 10.6, 8.6 and 10.1% increase in its protein contents respectively.

Feeding trials on undetoxified, aqueous extracted and enzymic hydrolysed RL-18 seed meal showed that 25% of the rats fed on undetoxified meal died during the experiment whereas those fed on aqueous extracted and enzymically detoxified meals showed an increase in body weight of 130 and 180 g respectively over a period of 7 weeks.

Proteins present in the supernatant were added to aqueous extracted or enzymically detoxified meal after pan-drying, spray drying or after steam coagulation. Rats fed on diet containing aqueous detoxified cake plus pan-dried supernatant showed 88% loss of hair. Supplementation of aqueous extracted RL-18(Mustard seed) cake with pan-dried supernatant suppressed the growth of rats but weight gain (190 g.) was comparatively more than the diet containing RL-18 detoxified cake as such which showed 120 g. gain in body weight. Aqueous extracted RL-18 cake supplemented with proteins coagulated from the supernatant by passing steam when incorporated into the diet showed high rate of growth, maximum weight gain (255 g) and minimum loss of body hair (16%). The feed containing enzymically detoxified meal plus steam coagulated proteins of supernatant showed a significant increase in growth rate, NPU and PER values and checked the loss of body hair of rats. Rats fed on biologically detoxified RL-18 cake plus roller drum-dried supernatant after steam stripping showed maximum growth rate, NPU and PER values among other experimental diets Detoxification of RL-18 cake with Aspergillus flavus increased its fibre content to 16%. However the process of enzymic hydrolysis was found to be the best among chemical and biochemical methods of detoxification of seed/cake

Download Full Thesis
1701.03 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
116.06 KB
2 1 Introduction 1
78.09 KB
3 2 Review of literature 6
78.09 KB
4 3 Experimental 45
130.89 KB
  3.1 Materials and methods 45
  3.2 Analytical 55
  3.3 Composition of vitamin Mixtural 59
  3.4 Composition of Mineral mixture 59
  3.5 Composition of trace salts 60
5 4 Results 61
372.27 KB
6 5 Discussion on Results 144
371.62 KB
7 6 Summary 163
220.93 KB
8 7 Bibliography 166
360.34 KB
9 8 Appendix 180
26.72 KB