I= BIOSYNTHESIS OF GLUCOSE ISOMERASE BY STREPTOMYCES SPECIES
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Title of Thesis
BIOSYNTHESIS OF GLUCOSE ISOMERASE BY STREPTOMYCES SPECIES

Author(s)
Owais Yunus
Institute/University/Department Details
Institute of Chemistry/ University of the Punjab
Session
Subject
Chemistry
Number of Pages
175
Keywords (Extracted from title, table of contents and abstract of thesis)
glucose isomerase, streptomyces species, dilution plantes, fructose assay

Abstract
No Abstract

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1459.76 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
83.5 KB
2 1 Introduction 1
79.29 KB
3 2 Review Of Literature 8
270.31 KB
4 3 Materials And Methods 30
715.96 KB
  3.1 Materials 30
  3.2 Methods 31
  3.3 Isolation Of Streptomyces Species 31
  3.4 Preparation Of Dilution Plantes 31
  3.4 Screening Test 32
  3.5 Fructose Assay 33
  3.6 Fermentation Studies 33
  3.7 Organism Used 33
  3.8 Stock Culture 33
  3.9 Vegetative Growth Inoculum 33
  3.10 Studies In Shake Flask 34
  3.11 Experiments In Ferments 34
  3.12 Extraction And Preparation Of Cured Enzyme 35
  3.13 Partial Purification Of Glucose Isomerase 35
  3.14 Preparation Of Cell Bound Enzyme 36
  3.15 Preparation Of Batch Reactor 36
  3.16 Initial Rates 37
  3.17 Estimation Of Protein 38
  3.18 Estimation Of Protein 38
  3.19 Estimation Of Cell Dry Weight 38
  3.20 Effect Of Ph On Enzyme Activity 39
  3.21 Effect Of Temperature 39
  3.22 Thermal Tolerance And Ph Stability Of Glucose Isomerase 39
  3.23 Determination Of Equilibrium Constants 39
  3.24 Continuation Use Of Fixed Isomerase 40
  32.5 Preservation Of Cell Mass 40
  3.26 Preparation Of Hydrolyzate Of Corn Cobs 40
  3.27 Assay Of Glucose Isomerase Activity 41
  3.28 Enzyme Unit 41
  3.29 Specific Activity 41
  3.30 Results 42
5 4 Discussion 131
148.6 KB
6 5 Summary 148
35.6 KB
7 6 Bibliography 151
107.22 KB