I= ASSOCIATIVE BIOLOGICAL NITROGEN FIXATION IN PLANTS GROWING IN SALINE SOILS
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Title of Thesis
ASSOCIATIVE BIOLOGICAL NITROGEN FIXATION IN PLANTS GROWING IN SALINE SOILS

Author(s)
Rakhshanda Bilal
Institute/University/Department Details
University of the Punjab
Session
1998
Subject
Botany
Number of Pages
270
Keywords (Extracted from title, table of contents and abstract of thesis)
saline soils, nitrogen fixation, acetylene reduction assay, diazotrophs

Abstract
No Abstract

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2904.1 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
61.14 KB
2 1 Introduction And Literature Survey 1
407.39 KB
  1.1 Introduction 1
  1.2 Literature Survey 4
3 2 Materials And Methods 43
226.14 KB
  2.1 Survey Site 43
  2.2 Sampling 43
  2.3 Acetylene Reduction Assay (ARA) Technique 44
  2.4 Enumeration Of N 2 -Fixing Bacteria 47
  2.5 Isolation Procedure 49
  2.6 Identification 50
  2.7 Fluorescent Antibody Technique 50
  2.8 Seed Sterilization And Germination 55
  2.9 Long Glass Tube Assembly 56
  2.10 Fahraeus Slide Technique 57
  2.11 15 N Isotopic Dilution Technique 57
  2.12 Photography 59
4 3 Ecology Of Root Associated Diazotrophs 60
419.19 KB
  3.1 Introduction 60
  3.2 Root Associated N 2 -Ase Activity 62
  3.3 Enumeration Of Diazotrophs In Various Rhizosphere Fractions 74
  3.4 Discussion 83
5 4 Enrichment Isolation And Identification Of Root Associated Diazotrophs 93
331.08 KB
  4.1 Introduction 93
  4.2 N 2 -Ase Activity Of Enrichment Culture 96
  4.3 Isolation And Identification Of Diazotrophs 99
  4.4 Discussion 116
6 5 Physiology Of The Diazotrophs Isolated From The Grass Roots 127
490.43 KB
  5.0 Introduction 127
  5.1 C-Source Utilization For Growth And N 2 -Ase Activity 128
  5.2 Effect Of Basal Media On N 2 -Ase Activity Of Diazotrophs 138
  5.3 Effect Of Combined N On Growth And N 2 -Ase Activity Of Diazotrophs 158
7 6 Survival And Root Colonization Of Inoculated Diazotrophs 171
502.61 KB
  6.1 Introduction 171
  6.2 Attachment Of The Diazotrophs To Roots 173
  6.3 Root Colonization 178
  6.4 Survival And Colonization Studied In Soil System 190
  6.5 Quantification Of N 2 -Fixation Using 15 N 199
  6.6 Discussion 206
8 7 Summary 214
428.58 KB
  7.1 References 220
  7.2 Appendix 247