I= INVESTIGATION ON THE ISOLATES AND DERIVATIVES OF PANT ANTI-BACTERIALS
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Title of Thesis
INVESTIGATION ON THE ISOLATES AND DERIVATIVES OF PANT ANTI-BACTERIALS

Author(s)
Muhammad Tahir Javed Khan
Institute/University/Department Details
Faculty of Pharmacy University of the Punjab, Lahore
Session
1991
Subject
Pharmaceutical Chemistry
Number of Pages
210
Keywords (Extracted from title, table of contents and abstract of thesis)
PLANT ANTI-BACTERIALS, Ardisia Solanacea Roxb, A. solanacea, hexane, chloroform, ethanol extracts, Myrsine Africana Linn, Family myrsinaceae

Abstract
Ardisia Solanacea Roxb. In vitro antibacterial studies were performed successively with hexane, chloroform and ethanol extracts of leaves, stems and seeds of plant A. solanacea. All the extracts except of the hexane and chloroform extracts of stems and chloroform extract of seeds showed antibacterial activity against both gram-positive and gram-negative microorganisms. The antibacterial rage of ethanolic extracts wee more prominent than those of the chloroform and hexane.

Qualitative phytochemical tests on the above mentioned extracts indicated that the pant might contain alkaloids, steroids, resins, tannins as well as reducing sugars.

The hexane extract of leaves of A. solanacea was separated into various fraction characterized as hydrocarbons, esters, triterpendoidal alcohols, sterols and a polar mass which were designated as isolates. The antibacterial activity of these isolates, against gram-positive and gram-negative bacteria in vitro was compared with ampicillin and streptomycin

All the isolates except sterols exhibited antibacterial activity against some or all the bacterial studied. Among all the isolates the polar mass was broad spectrum and it exhibited maximum activity which was1/16th of the standard antibiotics used.

Myrsine Africana Linn. In vitro antibacterial studies were carried out successively with the hexane, chloroform, and ethanol extract except those of the hexane from stems and roots of the plant M. Africana. All the extracts except those of the hexane from stems and roots exhibited antibacterial activity against both gram-positive and gram-negative microorganisms. The chloroform extracts were more potent than the ethanolic extracts in this respect.

Qualitative phytochemical tests revealed that the plant might contain alkaloids, glycosides, steroids, reducing sugars, as well as tannins and resins. n-Alkyl hydroxybenzoquinones were isolated from the hexane extract of the stems of M. Africana and purified by physical means. To determine the homologues of the side chain, they were subjected to oxidation. The monocarboxylic acids obtained were esterifies and analyzed by gas chromatography

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1728.67 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
130.78 KB
2 1 Introduction 1
69.86 KB
3 2 Literature Survey 9
318.56 KB
  2.1 Family Myrsinaceae(Botanical Description) 9
4 3 Materials And Methods 54
162.26 KB
  3.1 Instruments 54
  3.2 Solvents 54
  3.3 Chemicals/Reagents 55
  3.4 Adsorbents 56
  3.5 Plant Material 57
  3.6 Preparation Of Extracts From Different Parts Of Ardisia Solanacea Roxb 57
  3.7 Preparation Of Isolates Form Hexane Extract Of Leaves Of Ardisia Solanacea 60
  3.8 Preparation Of Extract From Different Parts Of Myrsine Africana Linn 63
  3.9 Isolation Of The Coloring Matter From M Africana Stems 67
  3.10 Examination Of The Extracts Form Different Parts Of Ardisia Solanicea And Myrsine Africana 70
  3.11 Anti-Bacterial Activity Test 71
  3.12 References 75
5 4 Results 76
494.4 KB
  4.1 Phytochemical Investigations On Ardisia Solanacea 76
  4.2 Phytochemical Investigations On Myrsine Africana 88
  4.3 Anti-Bacteria Studies Of Ardisia Solanacea Extracts 95
  4.4 Anti-Bacterial Assay Of The Extracts From Myrsine Africana 116
6 5 Discussion 132
189.26 KB
7 6 Publication 154
334.87 KB