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Title of Thesis

Jahan Zeb Naz
Institute/University/Department Details
Department of Botany University of the Punjab, Lahore
Number of Pages
Keywords (Extracted from title, table of contents and abstract of thesis)
MYCORHIZA, SALT TOLERANCE, HELIANTHUS ANNUUS L. VAR. SUNCROSS, salinity, rhizospheric studies, Glomus fasciculatum , G. macrocarpum, G. mosseae, fungi, saline soil, mycorrhizal fungi

The project aims at finding a biological control of salinity by the introduction of appropriate mycorrhiza in plants under saline conditions. A umber of assays in pursuance of this goal were designed and performed:

During the rhizospheric studies of native halophytic vegetation, 32 different non-mycorrhizal fungal species were isolated, whereas a total of 8 mycorrhizal species were identified among which Glomus fasciculatum , G. macrocarpum and G. mosseae were more prevalent,

Salt tolerance assays of mycorrhizal isolates revealed maximum spore germination in basic culture medium. In comparative salt tolerance studies maximum spore germination was obtained in G. fasciculatum, minimum in G. mosseae and intermediate in G. macrocarpum,

alt tolerance assays of non-mycorrhizal fungal species showed that the species of Aspergillus and Mucor were highly; Alternaria-alternata and Penicillium sp., were moderately; and Curvularia clavata and Fusarium solani were the least salt tolerant species,

Interactions between mycorrhizal and non-mycorrhizal fungi under various salinity levels in pots indicated that the general response of the Glomus spp., was almost similar ;i.e there was a decrease in extent of VA mycorrhizal infection with an increase in salinity level. The plants inoculated with G. fasciculatum showed maximum, with G. macrocarpum intermediate and with G. mosseae least extent of VA mycorrhizal infection. Results of shoot dry weight and phosphorus content were also parallel to the results of extent of mycorrhizal infection. Sodium and chloride content of plants inoculated with G. fasciculatum and maximum number of non-mycorrhizal fungi was significantly lower as compared to rest of the treatments,

Evaluation of the performance of selected mycorrhizal and non-mycorrhizal fungi, under saline field conditions indicated that the mycorrhizal status and shoot dry weight was significantly greater in plants inoculated with saline VA mycorrhizal inoculum as compared to the plants inoculated with normal field spores or non-inoculated controls. Moreover, the sporulation of saline mycorrhiza was much higher as compared to normal field mycorrhiza.

Probable mechanisms involved in mycorrhiza induced salt tolerance and its economic feasibility are discussed.

Download Full Thesis
3233.45 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
81.51 KB
2 1 General Introduction 1
125.24 KB
3 2 Isolation Non- Mycorrhizal And Mycorrhizal Of Saline Soil And Mycorrhizal Studies On Native Halophytic 12
363.46 KB
  2.1 Introduction 12
  2.2 Materials And Methods 16
  2.3 Results 19
  2.4 Discussion 30
4 3 Viability And Germination Of Endomycorrhizal Spores 39
97.07 KB
  3.1 Introduction 39
  3.2 Materials And Methods 40
  3.3 Results 42
  3.4 Discussion 45
5 4 Salt Tolerance Assay Of Non- Mycorrhizal Isolates 48
157.2 KB
  4.1 Introduction 48
  4.2 Materials And Methods 49
  4.3 Results 54
  4.4 Discussion 54
6 5 Interactions Between Mycorrhizal And Non- Mycorrhizal Fungi Under Normal Soil Conditions 56
1030.74 KB
  5.1 Introduction 56
  5.2 Materials And Methods 59
  5.3 Results 65
  5.4 Discussion 110
7 6 Interactions Between Mycorrhizal And Non- Mycorrhizal Fungi Under Various Salinity Levels 114
426 KB
  6.1 Introduction 114
  6.2 Material And Methods 114
  6.3 Results 116
  6.4 Evaluation Of The Performance Of Selected Mycorrhizal Fungi Under Saline Filed Conditions 149
8 7 Evaluation Of The Performance Of Selected Mycorrhizal And Non- Mycorrhizal Fungi Under Saline Filed Conditions 149
260.97 KB
  7.1 Introduction 149
  7.2 Material And Methods 149
  7.3 Results 151
  7.4 Discussion 158
9 8 General Discussion 163
99.4 KB
10 9 References 171
563.09 KB
  9.1 Appendices