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CLINICAL INVESTIGATION AND DIAGNOSIS OF MALARIAL PARASITES AND THEIR VECTORS AMONG POPULATION OF KARACHI ON DISTRICT LEVEL CORRELATED WITH ENVIRONMENTAL AND HOME MANAGEMENT

Syed, Azra Qamar (2005) CLINICAL INVESTIGATION AND DIAGNOSIS OF MALARIAL PARASITES AND THEIR VECTORS AMONG POPULATION OF KARACHI ON DISTRICT LEVEL CORRELATED WITH ENVIRONMENTAL AND HOME MANAGEMENT. PhD thesis, University of Karachi, Karachi.

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Abstract

A total of 3821 males and females from different districts of Karachi and 2347 males and females from different Goths were analysed for malarial parasites (Plasmodium falciparum, P. vivax, P. ovale and P. malariae) harboring in their blood along with their vectors (5194) Anopheles including A. stephensi, A. culicifacies, A. subpictus, A. fluviatilis and A. pulcherimus. Correlation was carried out at the University of Arizona, USA for the association of parasites with the vector using PCR confirmatory test. This test proved that P. flaciparum and P. vivax were the species responsible for the malarial infection in the area of study Similarly A. stephensi, A. culicifacies and A. subpictus were proved to be actual carriers of malaria in Karachi city and their suburbs. A correlation of the vectors and parasites was also made with environmental changes, seasonal variation and home management system Incidence of P. falciparum was 60.43%, 33.82% and 49.43%, of P. vivax was 39.56% 66.72% and 59.56% in 2002, 2003 and 2004 respectively from Karachi city, of P. falciparum 56.33% 57.73% and 59.42% of P. vivax was 43.66%, 42.26% and 40.57% from different Goths. Whereas occurrence of A.stephensi was 15.10% in 2002, 31.16% in 2003 and 38.46% in 2004, of A. culicifacies was 58.54% 50.59% and 42.07% in 2002, 2003 and 2004 in turn A highly sensitive nested polymerase chain reaction was applied on the different species of dried Anopheles mosquitoes collected from Karachi, Pakistan and adjacent Goths, by using simple DNA extraction for the detection of sporozoites, at the University of Arizona, Tueson, Arizona, USA. This experiment revealed that 38% pools of A. stephensi and 33% pools of A. culicifacies were positive for plasmodium. In contrast only 9% were positive in A. culicifacies were positive in A. subpictus whereas A. fluviatilis and A. pulcherimus were found to be negative. Only 12% A. stephensi harboured had mixed infection but only 8% A. culicifacies had mixed infection. The ratio of P. falciparum, and P. vivax infection was 20:5 in A. stephensi and 10:16 in A. culicifacies whereas in A. subpictus, it was 3:6 in the above mentioned orders The current investigation reveals that P. vivax was the main cause of malaria in Karachi city and P. falciparum was on the peak in Goths. Among the Anopheles A. culicifacies was the most prominent vector reported from Karachi and suburbs. Important factors such as environmental effects, and home management systems are also briefly discussed. Notable health remedies against this persistent disease in Karachi city and Goths are also suggested

Item Type:Thesis (PhD)
Uncontrolled Keywords:malarial parasites, karachi, plasmodium falciparum, p. vivax, p. ovale, p. malariae, anopheles, a. stephensi, a. culicifacies, a. subpictus, a. fluviatilis, a. pulcherimus
Subjects:Biological & Medical Sciences (c) > Biological Sciences(c1) > Paleo-zoology(c1.10)
ID Code:1405
Deposited By:Mr. Muhammad Asif
Deposited On:26 Feb 2007
Last Modified:04 Oct 2007 21:06

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