I= DIAGNOSIS, IMMUNE RESPONSE AND ROLE OF CYTOKINES IN HELICOBACTER PYLORI ASSOCIATED GASTRO DUODENAL ULCER DISEASE
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Title of Thesis
DIAGNOSIS, IMMUNE RESPONSE AND ROLE OF CYTOKINES IN HELICOBACTER PYLORI ASSOCIATED GASTRO DUODENAL ULCER DISEASE

Author(s)
Muhammad Amjad
Institute/University/Department Details
University Of Karachi/ Department Of Microbiology
Session
1997
Subject
Microbiology
Number of Pages
179
Keywords (Extracted from title, table of contents and abstract of thesis)
cytokines, helicobacter pylori, gastro duodenal ulcer, h. pylori

Abstract
No Abstract

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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
286.81 KB
2 1 Introduction 1
30.66 KB
3 2 Aims And Objectives 3
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4 3 Literature Review 5
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  3.1 Helicobacter Pylori As A Human Pathogen And Its Role In Gastro duodenal Ulcer Disease 5
  3.2 Microbiological Considerations Of Helicobacter Pylori 6
  3.3 Epidemiology Of Helicobacter Pylori Infection 8
  3.4 Pathogenic Mechanisms Of Helicobacter Pylori Infection 9
  3.5 Determinants Of Pathogenecity Of H. Pylori 9
  3.6 Helicobacter Pylori Infection And Gastric Cancer 16
  3.7 Effects Of Helicobacter Pylori Infection 17
  3.8 Immune Response To Helicobacter Pylori Infection 18
  3.9 Diagnosis Of Helicobacter Pylori Infection 19
  3.10 Treatment Of H. Pylori Infection 19
  3.11 Cytokines: General Review 20
  3.12 Features Of Cytokines Involved In Bacterial Infections 21
5 4 Materials And Methods 37
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  4.1 Endoscopic Examination Of Patients 37
  4.2 Collection Of Biopsy Specimens 40
  4.3 Transport Of Biopsy Specimens 40
  4.4 Processing Of Biopsy Specimens For Culture Of H. Pylori 40
  4.5 Rapid Helicourease Test For The Diagnosis Of H. Pylori Infection 46
  4.6 Preparation Of Tubes For Helicourease Test 46
  4.7 Comparison Of Helicourease And Urea Broth 47
  4.8 Histopathological Study Of Biopsies For H. Pylori Associated Gastritis 47
  4.9 Development Of Serological Tests For The Diagnosis Of H. Pylori Infection 51
  4.10 Determination Of Serum Immunoglobulin Level In Gastroduodenal Ulcer And H. Pylori Infected Patients 56
  4.11 Determination Of C-Reactive Protein In Gastroduodenal Ulcer And H.Pylori Infected Patients 57
  4.12 The Role Of Cytokines In The Immunopathogeneis Of Helicobacter Pylori Associated Gastroduodenal Ulcer Disease 58
  4.13 Statistical Analysis Of Data 70
6 5 Results 71
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  5.1 Morphological Characters Of H. Pylori Isolates 74
  5.2 Cultural Characters Of H. Pylori Isolates 74
  5.3 Biochemical Characters Of H. Pylori Isolates 74
  5.4 Tests For The Presumptive Diagnosis Of H. Pylori Infection: Helicourease Test & Giemsa’s Staining Of Biopsy Tissues 74
  5.5 Prevalence Of H. Pylori Infection In Karachi, Pakistan 76
  5.6 Prevalence Of H. Pylori Infection Sex Distribution 76
  5.7 Relation Of H. Pylori Infection With Age Groups 76
  5.8 Symptoms Of Patients With H. Pylori Infection 79
  5.9 Association Of H. Pylori Infection With Gastroendoscopic Findings 79
  5.10 Isolation Of H. Pylori From Different Gastrointestinal Sites 79
  5.11 Association Of H. Pylori Infection With Socioeconomic Status And Habitats 82
  5.12 Antibiotic Susceptibility Pattern Of H. Pylori Isolates 82
  5.13 Hisotpathological Study Of H. Pylori Associated Gastroduodenal Ulcer Disease 85
  5.14 Comparison Of Tube Urease And Helicourease Test 91
  5.15 Comparison Of Culture, Histology And Helicourease Method For The Diagnosis Of H. Pylori Infection 91
  5.16 Seroprevalence Of H. Pylori Infection In Karachi, Pakistan : Non-Invasive Serological Tests For The Diagnosis Of H. Pylori Infection 93
  5.17 Comparison Of Microagglutination , Thin Layer Immunoassay, Elisa And Dot Elisa For Serological Diagnosis Of H. Pylori Infection 97
  5.18 Serum Immunoglobulin Level In Gastroduodenal Ulcer And H. Pylori Infected Patinets 101
  5.19 C-Reactive Protein In Gastroduodenal Ulcer And H. Pylori Infected Patients 104
  5.20 Release Of Il- Iβ , Il-6 And Tnf -A From Human Gastric Tissues Of H. Pylori Positive And Negative Patients 107
  5.21 Effect Of Il-4, Il-6 And Ifn -Y On The Production Of Il- Ib , Il-6 And Tnf -A By The Cultured Human Gastric 108
  5.22 Release Of Il-2, Il-4 And Ifn -Y From H. Pylori Stimulated Human Peripheral Blood Lymphocytes 111
  5.23 Effect Of Il- Ib , Il-6 And Tnf -A On The Production Of Il-2, Il-4 And Ifn -Y By H. Pylori Stimulated Human Peripheral Blood Lymphocytes 113
7 6 Discussion 116
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  6.1 Prevalence Of Helicobacter Pylori Infection In Karachi, Pakistan 116
  6.2 Factors Affecting The Prevalence Of Helicobacter Pylori Infection 118
  6.3 Seroprevalence Helicobacter Pylori Infection In Karachi Pakistan 119
  6.4 Histopathological Diagnosis Of H. Pylori And Role Of H. Pylori In Gastric Cancer 122
  6.5 Comparison Of Invasive And Non-Invasive Tests For The Diagnosis Of H. Pylori Infection 125
  6.6 Role Of Cytokines In The Pathogenic And Immune Mechanism Of Helicobactger Pylori Infection 129
8 7 Conclusions 137
29.67 KB
9 8 References 139
388.26 KB
10 9 Appendix 167
36.9 KB