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| Pakistan Research Repository | Home |
Title of Thesis |
| Author(s) Muhammad Amjad |
| Institute/University/Department Details University Of Karachi/ Department Of Microbiology |
| Session 1997 |
| Subject Microbiology |
| Number of Pages 179 |
| Keywords (Extracted from title, table of contents and abstract of thesis) cytokines, helicobacter pylori, gastro duodenal ulcer, h. pylori |
Abstract |
| Download Full Thesis |  2289.18 KB |
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| S. No. | Chapter | Title of the Chapters | Page | Size (KB) |
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| 1 | 0 | Contents | 286.81 KB |
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| 2 | 1 | Introduction | 1 | 30.66 KB |
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| 3 | 2 | Aims And Objectives | 3 | 27.96 KB |
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| 4 | 3 | Literature Review | 5 | 353.61 KB |
| 3.1 | Helicobacter Pylori As A Human Pathogen And Its Role In Gastro duodenal Ulcer Disease | 5 | ||
| 3.2 | Microbiological Considerations Of Helicobacter Pylori | 6 | ||
| 3.3 | Epidemiology Of Helicobacter Pylori Infection | 8 | ||
| 3.4 | Pathogenic Mechanisms Of Helicobacter Pylori Infection | 9 | ||
| 3.5 | Determinants Of Pathogenecity Of H. Pylori | 9 | ||
| 3.6 | Helicobacter Pylori Infection And Gastric Cancer | 16 | ||
| 3.7 | Effects Of Helicobacter Pylori Infection | 17 | ||
| 3.8 | Immune Response To Helicobacter Pylori Infection | 18 | ||
| 3.9 | Diagnosis Of Helicobacter Pylori Infection | 19 | ||
| 3.10 | Treatment Of H. Pylori Infection | 19 | ||
| 3.11 | Cytokines: General Review | 20 | ||
| 3.12 | Features Of Cytokines Involved In Bacterial Infections | 21 | ||
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| 5 | 4 | Materials And Methods | 37 | 503.72 KB |
| 4.1 | Endoscopic Examination Of Patients | 37 | ||
| 4.2 | Collection Of Biopsy Specimens | 40 | ||
| 4.3 | Transport Of Biopsy Specimens | 40 | ||
| 4.4 | Processing Of Biopsy Specimens For Culture Of H. Pylori | 40 | ||
| 4.5 | Rapid Helicourease Test For The Diagnosis Of H. Pylori Infection | 46 | ||
| 4.6 | Preparation Of Tubes For Helicourease Test | 46 | ||
| 4.7 | Comparison Of Helicourease And Urea Broth | 47 | ||
| 4.8 | Histopathological Study Of Biopsies For H. Pylori Associated Gastritis | 47 | ||
| 4.9 | Development Of Serological Tests For The Diagnosis Of H. Pylori Infection | 51 | ||
| 4.10 | Determination Of Serum Immunoglobulin Level In Gastroduodenal Ulcer And H. Pylori Infected Patients | 56 | ||
| 4.11 | Determination Of C-Reactive Protein In Gastroduodenal Ulcer And H.Pylori Infected Patients | 57 | ||
| 4.12 | The Role Of Cytokines In The Immunopathogeneis Of Helicobacter Pylori Associated Gastroduodenal Ulcer Disease | 58 | ||
| 4.13 | Statistical Analysis Of Data | 70 | ||
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| 6 | 5 | Results | 71 | 592.7 KB |
| 5.1 | Morphological Characters Of H. Pylori Isolates | 74 | ||
| 5.2 | Cultural Characters Of H. Pylori Isolates | 74 | ||
| 5.3 | Biochemical Characters Of H. Pylori Isolates | 74 | ||
| 5.4 | Tests For The Presumptive Diagnosis Of H. Pylori Infection: Helicourease Test & Giemsa’s Staining Of Biopsy Tissues | 74 | ||
| 5.5 | Prevalence Of H. Pylori Infection In Karachi, Pakistan | 76 | ||
| 5.6 | Prevalence Of H. Pylori Infection Sex Distribution | 76 | ||
| 5.7 | Relation Of H. Pylori Infection With Age Groups | 76 | ||
| 5.8 | Symptoms Of Patients With H. Pylori Infection | 79 | ||
| 5.9 | Association Of H. Pylori Infection With Gastroendoscopic Findings | 79 | ||
| 5.10 | Isolation Of H. Pylori From Different Gastrointestinal Sites | 79 | ||
| 5.11 | Association Of H. Pylori Infection With Socioeconomic Status And Habitats | 82 | ||
| 5.12 | Antibiotic Susceptibility Pattern Of H. Pylori Isolates | 82 | ||
| 5.13 | Hisotpathological Study Of H. Pylori Associated Gastroduodenal Ulcer Disease | 85 | ||
| 5.14 | Comparison Of Tube Urease And Helicourease Test | 91 | ||
| 5.15 | Comparison Of Culture, Histology And Helicourease Method For The Diagnosis Of H. Pylori Infection | 91 | ||
| 5.16 | Seroprevalence Of H. Pylori Infection In Karachi, Pakistan : Non-Invasive Serological Tests For The Diagnosis Of H. Pylori Infection | 93 | ||
| 5.17 | Comparison Of Microagglutination , Thin Layer Immunoassay, Elisa And Dot Elisa For Serological Diagnosis Of H. Pylori Infection | 97 | ||
| 5.18 | Serum Immunoglobulin Level In Gastroduodenal Ulcer And H. Pylori Infected Patinets | 101 | ||
| 5.19 | C-Reactive Protein In Gastroduodenal Ulcer And H. Pylori Infected Patients | 104 | ||
| 5.20 | Release Of Il- Iβ , Il-6 And Tnf -A From Human Gastric Tissues Of H. Pylori Positive And Negative Patients | 107 | ||
| 5.21 | Effect Of Il-4, Il-6 And Ifn -Y On The Production Of Il- Ib , Il-6 And Tnf -A By The Cultured Human Gastric | 108 | ||
| 5.22 | Release Of Il-2, Il-4 And Ifn -Y From H. Pylori Stimulated Human Peripheral Blood Lymphocytes | 111 | ||
| 5.23 | Effect Of Il- Ib , Il-6 And Tnf -A On The Production Of Il-2, Il-4 And Ifn -Y By H. Pylori Stimulated Human Peripheral Blood Lymphocytes | 113 | ||
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| 7 | 6 | Discussion | 116 | 267.57 KB |
| 6.1 | Prevalence Of Helicobacter Pylori Infection In Karachi, Pakistan | 116 | ||
| 6.2 | Factors Affecting The Prevalence Of Helicobacter Pylori Infection | 118 | ||
| 6.3 | Seroprevalence Helicobacter Pylori Infection In Karachi Pakistan | 119 | ||
| 6.4 | Histopathological Diagnosis Of H. Pylori And Role Of H. Pylori In Gastric Cancer | 122 | ||
| 6.5 | Comparison Of Invasive And Non-Invasive Tests For The Diagnosis Of H. Pylori Infection | 125 | ||
| 6.6 | Role Of Cytokines In The Pathogenic And Immune Mechanism Of Helicobactger Pylori Infection | 129 | ||
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| 8 | 7 | Conclusions | 137 | 29.67 KB |
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| 9 | 8 | References | 139 | 388.26 KB |
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| 10 | 9 | Appendix | 167 | 36.9 KB |
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