I= ISOLATION, CHARACTERIZATION OF FATTY ACIDS IN MARINE FISHES AND STUDY OF THEIR HYPOLIPIDEMIC EFFECTS IN COMPARISON WITH GEMFIBROZIL AND RELATED COMPOUNDS
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Title of Thesis
ISOLATION, CHARACTERIZATION OF FATTY ACIDS IN MARINE FISHES AND STUDY OF THEIR HYPOLIPIDEMIC EFFECTS IN COMPARISON WITH GEMFIBROZIL AND RELATED COMPOUNDS

Author(s)
Sohail Hassan
Institute/University/Department Details
Department of Pharmaceutical Chemistry/ University of Karachi
Session
2002
Subject
Pharmacy
Number of Pages
264
Keywords (Extracted from title, table of contents and abstract of thesis)
fatty acids, fish, hypolipidemic effects, gemfibrozil, omega-3, fish oil, docosahexaenoic acid, eicosapentaenoic acid, hyperlipidemia, lipids

Abstract
Omega-3 fatty acids are the most important constituent of fish oil, which showed significant activity against various clinical complications. To explore the possibility of obtaining docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA),a study on oils from six species of marine fish of coastal water of Karachi is carried out. The fatty acid composition of these fish were isolated, identified and characterized by thin layer chromatography, gas chromatography and gas chromatography-mass spectroscopy (GC-Ms). The total lipid contents in fish liver oil ranges from 5.03 to 20.25% of wet tissue. Among the course of our study twenty five (25) individual fatty acids are found in the fish oil. The analysis exhibited the presence of saturated fatty acid myristic, plamitic and static acid as major whereas lauric, pentadecanoic acid as minor constituents. Among unsaturated fatty acid monoenoic e.g. plamitoleic acid and oleic acid are the major component. Traces of dienoic and trienoic fatty acids are detected. It is interesting that polyunsaturated fatty acids EPA and DHA are also identified.

Biologically important fish oil and its constituents are studied in detail with special reference to cardiac aliments. The discovery of ω-3 fatty acid led to detail investigation about its effect of fish oil alone and in combination with fibric acid derivatives e.g gemfibrozil, fenofibrate and bezafibrate is found to be a potent cholesterol and triglyceride lowering agent.

It is suggested that these studies will lead to the formulation of effective therapeutic combinations with reduced side effects and toxicity . it is proposed that open chain fatty acid could inhibit the activity of enzymes which are responsible for the production of cholesterol and triglyceride in biological system to produce a blocking action by interaction or neutralization of the enzyme.

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1751.64 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
80.55 KB
2 1 Introduction 3
524.88 KB
  1.1 Composition Of Fish Oil 3
  1.2 Origin Of Fish Oil 5
  1.3 Biogenesis Of Fatty Acids 6
  1.4 Hyperlipidemia And Its Classification 8
  1.5 Treatment Of Hyperlipidemia 12
  1.6 Medicinal Importance 22
  1.7 Analytical Tools Applied For The Analysis Of Fish Oil 60
3 2 Experimental Work 70
222.73 KB
  2.1 Reagents And Other Chemicals 70
  2.2 Fish Used 70
  2.3 Collection Of Samples 75
  2.4 Extraction Of Lipids 75
  2.5 Qualitative Determination 76
  2.6 Biochemical Studies 82
4 3 Results And Discussion 99
791.73 KB
  3.1 Extraction And Preliminary Quantitative 99
  3.2 Detailed Qualitative And Quantitative Analysis Of Total Lipids (Fish Oil) 102
  3.3 Biochemical Studies 165
5 4 General Discussion 210
68.89 KB
6 5 Conclusion 218
42.29 KB
7 6 Bibliography 223
334.77 KB