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ISOLATION, CHARACTERIZATION OF FATTY ACIDS IN MARINE FISHES AND STUDY OF THEIR HYPOLIPIDEMIC EFFECTS IN COMPARISON WITH GEMFIBROZIL AND RELATED COMPOUNDS

Hassan, Sohail (2002) ISOLATION, CHARACTERIZATION OF FATTY ACIDS IN MARINE FISHES AND STUDY OF THEIR HYPOLIPIDEMIC EFFECTS IN COMPARISON WITH GEMFIBROZIL AND RELATED COMPOUNDS. PhD thesis, University of Karachi, Karachi.

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Abstract

Omega-3 fatty acids are the most important constituent of fish oil, which showed significant activity against various clinical complications. To explore the possibility of obtaining docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA),a study on oils from six species of marine fish of coastal water of Karachi is carried out. The fatty acid composition of these fish were isolated, identified and characterized by thin layer chromatography, gas chromatography and gas chromatography-mass spectroscopy (GC-Ms). The total lipid contents in fish liver oil ranges from 5.03 to 20.25% of wet tissue. Among the course of our study twenty five (25) individual fatty acids are found in the fish oil. The analysis exhibited the presence of saturated fatty acid myristic, plamitic and static acid as major whereas lauric, pentadecanoic acid as minor constituents. Among unsaturated fatty acid monoenoic e.g. plamitoleic acid and oleic acid are the major component. Traces of dienoic and trienoic fatty acids are detected. It is interesting that polyunsaturated fatty acids EPA and DHA are also identified. Biologically important fish oil and its constituents are studied in detail with special reference to cardiac aliments. The discovery of ω-3 fatty acid led to detail investigation about its effect of fish oil alone and in combination with fibric acid derivatives e.g gemfibrozil, fenofibrate and bezafibrate is found to be a potent cholesterol and triglyceride lowering agent. It is suggested that these studies will lead to the formulation of effective therapeutic combinations with reduced side effects and toxicity . it is proposed that open chain fatty acid could inhibit the activity of enzymes which are responsible for the production of cholesterol and triglyceride in biological system to produce a blocking action by interaction or neutralization of the enzyme.

Item Type:Thesis (PhD)
Uncontrolled Keywords:fatty acids, fish, hypolipidemic effects, gemfibrozil, omega-3, fish oil, docosahexaenoic acid, eicosapentaenoic acid, hyperlipidemia, lipids
Subjects:Biological & Medical Sciences (c) > Medical Sciences (c2) > Pharmacy(c2.5)
ID Code:1376
Deposited By:Mr. Muhammad Asif
Deposited On:19 Feb 2007
Last Modified:04 Oct 2007 21:06

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