I= STUDIES IN THE BIOCHEMISTRY OF MICROORGANISMS ISOLATION AND ELUCIDATION OF STRUCTURE OF METABOLITES, FROM ASPERGILLUS STELLATUS –CURZI PENICILLIUM MARTENSII-BIOURGE
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Title of Thesis
STUDIES IN THE BIOCHEMISTRY OF MICROORGANISMS ISOLATION AND ELUCIDATION OF STRUCTURE OF METABOLITES, FROM ASPERGILLUS STELLATUS –CURZI PENICILLIUM MARTENSII-BIOURGE

Author(s)
Naheed Akhtar
Institute/University/Department Details
University of Karachi
Session
Subject
Chemistry
Number of Pages
136
Keywords (Extracted from title, table of contents and abstract of thesis)
xanthones, mannitol, metabolites, aspergillus stellatus, penicillium martensii, shahenxanthone, najamxanthone

Abstract
From the mycelium of Aspergillus stellatus –Curzi besides mannitol, six new xanthones have been isolated. The proposed names, molecular formulae and the m.ps are tabled below:-

i)Tajixanthoune;C25H26O6, m.p.157-158oC; [α] D25-80o ii)Shamixanthone;C25H26O5, m.p.153-154oC; [α] D25+6.6o iii)Ajamxanthone;C25H24O4, m.p.131-132oC; [α] D25+15.3o iv)Shahenxanthone;C25H28O7, m.p.163-164oC; [α] D25-19.2o v)Najamxanthone;C26H28O8, m.p.181-182oC; [α] D25-61.2o vi)Radixanthone;C26H30O9, m.p.201-202oC;--

Spectroscopic data and chemical evidence has shown Tajixanthone to be: 3,4,4a,14a-Tatrahydro-4, 8-dihydroxy-2,2,6,9,14a-pentamethyl-3-methylene-2H, 5H, 7H-pyrano [3,2:5,6] pyrano [3,2-b] xanthene 7-one*. Shamixanthone to have the structure : 1,2,3,3a,12, 12a-Hexhydro-1,9-dihydrioxy-11-(hydroxymethyl) -3,3,3a,38tetramethyl-2-methylene-10H-pentalene [2,1-b] xanthene-10-one. Ajamxanthone to be:-------------------------- 2,3,3a,12-Tetramethyl-2-hydroxy-11-(hydeoxymethyl)-3,3,3a-8-tetramethy-2-methylene -10H- pentaleno [2,1-b] xanthene-10 one**

Similarly the structures of shahenxanthone and Najamxanthone have been established through chemical degradation and spectroscopic studies as 3,4-Dighyre-2,7-dihydroxy-3-(1-hydroxy-2-isopropylallyl)-5-(hydromxymethy1)-2,8-dimethl-2H, 6H-pyrano [3,2-b] xantheme-6-one and 3,4-dihydro-2, 7-dihydroxy-3-(1-hydroxy-2-isopropylallyl) -2,8-dimethyl -2H, 6H-pyrano [3,2-b] xanthene -6-one 5 acetate respectively.

Penicilium martensii-Biourge when grown on a semi-synthetic medium produces a number of crystalline compounds viz: amudol, C7H8O3 C1, m.p. 146-147oC and an aromatic acid m.p. 175oC from broth, and amudane (also from broth),, C17H19O6C, m.p.195-196oC,--------------------------- orgostorol, C28H44O, m.p.168oC, m.p.163oC from mycelium. Amudane, amudene and anmjane are identified to be the known metabolites griseofulvin, dehydrogriseofulvin and dihygrogriseofulvin, and the aromatic acid as 6-methyl salicylic acid.

Download Full Thesis
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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
77.22 KB
2 1 Aspergillus Stellatus-Curzi 1
1507.71 KB
  1.1 Introduction 1
  1.2 Isolation Of The Metabolites 2
  1.3 Structural Studies Of The Metabolites 8
  1.4 Biosynthesis Of Xanthones 71
  1.5 Experimental 87
3 2 Penicillium Martensii - Biourge 110
217.07 KB
  2.1 Introduction 110
  2.2 Isolation Of The Metabolites 111
  2.3 Structural Studies Of The Metabolites 115
4 3 Experimental 125
142.54 KB
  3.1 Bibliography 125