I= STUDIES REGARDING PHYCOCHEMISTRY AND BIOCTIVITY OF SEVEN BLUE-GREEN AND THREE GREEN FRESHWATER ALGAE OF SINDH
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Title of Thesis
STUDIES REGARDING PHYCOCHEMISTRY AND BIOCTIVITY OF SEVEN BLUE-GREEN AND THREE GREEN FRESHWATER ALGAE OF SINDH

Author(s)
Naila Butt
Institute/University/Department Details
University of Karachi/ Department of Botany
Session
2004
Subject
Botany
Number of Pages
232
Keywords (Extracted from title, table of contents and abstract of thesis)
green freshwater algae, blue-green algae, algae, fatty acids, aphanothece pallida, aphanothece stagnina, nostoc ellipsosporum, arthrospira platensis, lynghya hieronymusii, lyngbya martensiana, gloeotrichia natans, pithophora oedogonia, niteila flexilis

Abstract
Ten speices of freshwater algae were collected from different areas in the Sindh province of Pakistan and taxonomically identified. Their methanol extracts were investigated phycochemically as well as from the viewpoint of their bioactivity. Altogether 49 different fatty acids were detected including 17 saturated and 32 unsaturated acids, by GLC and GC-MS techniques. In most of the species unsaturated fatty acids were found in a larger proportion than the saturated acids. I almitoleic algal extracts while pentadecylic, palraitic, myristolic and oleic acids were the next commonly occurring acids. The fatty acid compositions of the investigated spices were found to very from phylum to phylum, order, family to family, genus to genus and species to species β-sitosterol and trans-phytol were isolated from eight algal extracts and chemically elucidated by NMR-spectroscopic techniques. Their occurrence also varied from species to species. Extracts of seven algal species were tested against five Gram positive and six Gram negative bacterial, where bacteria of both the categories behaved similarly and to difference could be found out in their sensitivity. Eight algal extracts were tested against three facultative parasites, six plant highly susceptible against these extracts. The extracts obtained from six species showed 33 to 100% significant phytotoxicity. Extracts of all algae displayed non-significant results in brine-shrimp and insecticidal bioassays. Antitumour activity was shown by the extract of one algal species

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1745.79 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents 0
248.24 KB
2 1 Introduction 28
34.23 KB
3 2 Materials And Methods 32
181.89 KB
  2.1 Algal Material 33
  2.2 Isolation Of Fatty Acids 37
  2.3 Separation Of Sterols And Terpehes 41
  2.4 Instrumentation 42
  2.5 Bioactivity Studies 46
4 3 Results 52
1159.45 KB
  3.1 Aphanothece Pallida 56
  3.2 Aphanothece Stagnina ( Sprengel ) A Braun In Rabenhorst 72
  3.3 Nostoc Ellipsosporum ( Desmazieses ) 87
  3.4 Arthrospira Platensis ( Nordstedt ) Gomont 1892 95
  3.5 Lynghya Hieronymusii Lemmermann 1905 103
  3.6 Lyngbya Martensiana Meneghini Ex Gomont 119
  3.7 Gloeotrichia Natans (Hedwig) Rabenhorst 1847 Ex Bornet 136
  3.8 Enteromorpha Intestinalis (Linnaeus) Nees 1820 153
  3.9 Pithophora Oedogonia Wittrock ( Montagne ) 163
  3.10 Niteila Flexilis (Linnaeus) Ca Agardh 1824 177
5 4 Discussion 191
182.76 KB
  4.1 Fatty Acids 192
  4.2 Sterol & Terpene 197
  4.3 Antibacterial Activity 199
  4.4 Antifungal Activity 200
  4.4 Other Bioactivities 201
6 5 Acknowledgments 211
23.78 KB
7 6 Literature cited 214
179.08 KB