I= STUDIES ON THE DEVELOPMENT OF VACCINE USING INDIGENOUS STRAINS OF MYCOPLASMA GALLISPECTICUM
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Title of Thesis
STUDIES ON THE DEVELOPMENT OF VACCINE USING INDIGENOUS STRAINS OF MYCOPLASMA GALLISPECTICUM

Author(s)
Rozina Anwar
Institute/University/Department Details
University of Karachi/ Department of Microbiology
Session
2000
Subject
Microbiology
Number of Pages
240
Keywords (Extracted from title, table of contents and abstract of thesis)
mycoplasma gallispecticum, vaccine, mycoplasma, avian mycoplasma, mycoplasmosis, e. coli infections, m. gallisepticum

Abstract
No abstract

Download Full Thesis
2363 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents 0
166.22 KB
2 1 Summary 1
37.03 KB
3 2 Introduction 5
48.42 KB
4 3 Review Of Literature 10
563.46 KB
  3.1 An Introduction To Mycoplasma 10
  3.2 Classification And Nomenclature 12
  3.3 Growth Of Mycoplasma 14
  3.4 Cultivation Of Mycoplasma 24
  3.5 Laboratory Diagnosis Of Mycoplasmosis 26
  3.6 Pathogenicity Of Avian Mycoplasma 43
  3.7 Control Of Avian Mycoplasmosis 59
  3.8 Secondary Infections Due To E. Coli 65
  3.9 Control Of E. Coli Infections 67
5 4 Materials And Methods 71
513.68 KB
  4.1 Culture Media 71
  4.2 Storage Of Media 81
  4.3 Isolation Of Mycoplasma 81
  4.4 Identification Of Mycoplasma 82
  4.5 Preparation Of Immunofluorescent Conjugate 89
  4.6 Perparation Of Mg Antigen For The Agglutination Test 89
  4.7 Study Of Immunogenic Response Of Different Isolates Of M. Gallisepticum 96
  4.8 Pathogenicity Determination Of Different Isolates Of M. Gallisepticum 100
  4.9 Development Of Mg Vaccine 100
  4.10 Pathogenecity Revival Of Mg-S6 Strain 106
  4.11 Experimental Design For Mg Vaccination 107
  4.12 Evalustion Of Mg Vaccine Efficacy 107
  4.13 Studies On Secondary Infection By E. Coli 111
  4.14 Isolation Of E. Coli 111
  4.15 Identification And Preservation Of E. Coli 112
  4.16 Pathogenecity Determination Of E. Coli 112
  4.17 Study Of The Secondary Bacterial Complications Produced By E. Coli In Experimental Mycoplasmosis In Chickens 116
  4.18 Evaluation Of The Bacterial Complications By E. Coli 117
  4.19 Development Of E. Coli Vaccine 119
  4.20 Experimental Design For E. Coli Vaccination 120
  4.21 Evaluation Of E. Coli Vaccine Effecacy 121
6 5 Results And Discussion 124
937.71 KB
  5.1 Cultre Media 124
  5.2 Isolation Of Mycoplasma 135
  5.3 Identification Of Mycoplasma 136
  5.4 Inhibition Of Autoagglutination Of Mg Antigen 140
  5.5 Stability Of Mg Antigen 143
  5.6 Study Of Immunogenic Response Of Different Isolates Of M. Gallisepticum 146
  5.7 Pathogenicity Of Mycoplasma Isolates 149
  5.8 Development Of M. Gallisepticum Vaccine 157
  5.9 Evaluation Of Vaccine Effecacy 160
  5.10 Pathogenecity Of E. Coli Isolates 179
  5.11 Evaluation Of The Secondary Bacterial Complications Produced By E. Coli 184
  5.12 Evaluation Of E. Coli Vaccine 195
7 6 References 205
391.21 KB
  5.1 Appendix 236